验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 5 publications below |
| IF | See 2 publications below |
产品信息
16871-1-AP targets AMOTL1 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | angiomotin like 1 |
| 别名 | AMOTL1, angiomotin like 1, Angiomotin like protein 1, JEAP |
| 计算分子量 | 107 kDa |
| 观测分子量 | 107 kDa |
| GenBank蛋白编号 | NM_130847 |
| 基因名称 | AMOTL1 |
| Gene ID (NCBI) | 154810 |
| RRID | AB_2305483 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8IY63 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The protein encoded by this gene is a peripheral membrane protein that is a component of tight junctions or TJs. TJs form an apical junctional structure and act to control paracellular permeability and maintain cell polarity. This protein is related to angiomotin, an angiostatin binding protein that regulates endothelial cell migration and capillary formation.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for AMOTL1 antibody 16871-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Curr Biol Cellular mechanotransduction relies on tension-induced and chaperone-assisted autophagy. | ||
Environ Toxicol Angiomotin-like 1 plays a tumor-promoting role in glioma by enhancing the activation of YAP1 signaling. | ||
Pulm Pharmacol Ther Mitigation of tight junction protein dysfunction in lung microvascular endothelial cells with pitavastatin. | ||
Cell Death Dis SRSF3/AMOTL1 splicing axis promotes the tumorigenesis of nasopharyngeal carcinoma through regulating the nucleus translocation of YAP1 | ||
Int J Biol Macromol Harnessing machine learning and multi-omics to explore tumor evolutionary characteristics and the role of AMOTL1 in prostate cancer |
