验证数据展示
经过测试的应用
Positive WB detected in | A549 cells, DU 145 cells, Saos-2 cells, NCI-H1299 cells, MDA-MB-468 cells, SK-BR-3 cells |
Positive IF/ICC detected in | A549 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:2000-1:10000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
68401-1-Ig targets AMOTL2 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.
经测试应用 | WB, IF/ICC, ELISA Application Description |
经测试反应性 | Human |
免疫原 | AMOTL2 fusion protein Ag19971 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | angiomotin like 2 |
别名 | AMOTL2, angiomotin like 2, Angiomotin like protein 2, KIAA0989, LCCP, Leman coiled coil protein |
计算分子量 | 779 aa, 86 kDa |
观测分子量 | 100 kDa |
GenBank蛋白编号 | BC011454 |
基因名称 | AMOTL2 |
Gene ID (NCBI) | 51421 |
RRID | AB_3085118 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein G purification |
UNIPROT ID | Q9Y2J4 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Angiomotin‑like 2 (AMOTL2) is a member of the motin family of angiostatin‑binding proteins. The motin family, also known as AMOTs, consists of three members: AMOT, AMOT-like 1 (AMOTL1) and AMOTL2. Members of the motin family are a type of adaptor proteins mainly distributed in the cytomembrane, cytoplasm or nucleus, and have a higher expression in the endothelial cells of capillaries and in larger vessels of the placenta. Human AmotL2 encodes two isoforms of a molecular mass of 100 kDa and 60 kDa.(PMID: 34036399,PMID: 25080976)
实验方案
Product Specific Protocols | |
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WB protocol for AMOTL2 antibody 68401-1-Ig | Download protocol |
IF protocol for AMOTL2 antibody 68401-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |