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AMOTL2 Monoclonal antibody

AMOTL2 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2C4A4

Cat no : 68401-1-Ig

Print datasheet

Synonyms

AMOTL2, angiomotin like 2, Angiomotin like protein 2, KIAA0989, LCCP, Leman coiled coil protein

验证数据展示

  • WB analysis using 68401-1-Ig

    WB analysis using 68401-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68401-1-Ig (AMOTL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 68401-1-Ig

    IF Staining of A549 using 68401-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using AMOTL2 antibody (68401-1-Ig, Clone: 2C4A4 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inA549 cells, DU 145 cells, Saos-2 cells, NCI-H1299 cells, MDA-MB-468 cells, SK-BR-3 cells
Positive IF/ICC detected inA549 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68401-1-Ig targets AMOTL2 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human
Immunogen AMOTL2 fusion protein Ag19971 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name angiomotin like 2
Synonyms AMOTL2, angiomotin like 2, Angiomotin like protein 2, KIAA0989, LCCP, Leman coiled coil protein
Calculated Molecular Weight 779 aa, 86 kDa
Observed Molecular Weight100 kDa
GenBank Accession NumberBC011454
Gene Symbol AMOTL2
Gene ID (NCBI) 51421
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9Y2J4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Angiomotin‑like 2 (AMOTL2) is a member of the motin family of angiostatin‑binding proteins. The motin family, also known as AMOTs, consists of three members: AMOT, AMOT-like 1 (AMOTL1) and AMOTL2. Members of the motin family are a type of adaptor proteins mainly distributed in the cytomembrane, cytoplasm or nucleus, and have a higher expression in the endothelial cells of capillaries and in larger vessels of the placenta. Human AmotL2 encodes two isoforms of a molecular mass of 100 kDa and 60 kDa.(PMID: 34036399,PMID: 25080976)

实验方案

Product Specific Protocols
WB protocol for AMOTL2 antibody 68401-1-IgDownload protocol
IF protocol for AMOTL2 antibody 68401-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols