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AMPD2 Polyclonal antibody

AMPD2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15710-1-AP

Print datasheet

Synonyms

AMP deaminase 2, AMP deaminase isoform L, AMPD2

验证数据展示

  • WB analysis of human liver using 15710-1-AP

    WB analysis of human liver using 15710-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 15710-1-AP (AMPD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human cerebellum using 15710-1-AP

    IHC staining of human cerebellum using 15710-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 15710-1-AP (AMPD2 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human cerebellum using 15710-1-AP

    IHC staining of human cerebellum using 15710-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 15710-1-AP (AMPD2 Antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inhuman liver tissue
Positive IHC detected inhuman cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

15710-1-AP targets AMPD2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen AMPD2 fusion protein Ag8335 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name adenosine monophosphate deaminase 2 (isoform L)
Synonyms AMP deaminase 2, AMP deaminase isoform L, AMPD2
Calculated Molecular Weight879aa,101 kDa; 798aa,92 kDa
Observed Molecular Weight 101 kDa
GenBank Accession NumberBC007711
Gene Symbol AMPD2
Gene ID (NCBI) 271
RRIDAB_2242657
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ01433
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Adenosine monophosphate deaminase (AMPD) catalyzes the deamination of AMP to IMP and plays an important role in the purine nucleotide cycle. Three AMPDs are present in mammals named AMPD1/2/3. AMPD2 is the main activity present in adult human liver and is widely expressed in non-muscle tissues and cells (PMID: 8526848). AMPD2 has some isoforms with the molecular mass of 88-101 kDa.

实验方案

Product Specific Protocols
WB protocol for AMPD2 antibody 15710-1-APDownload protocol
IHC protocol for AMPD2 antibody 15710-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

World J Clin Cases

Overexpression of AMPD2 indicates poor prognosis in colorectal cancer patients via the Notch3 signaling pathway.

Authors - Qi-Zhong Gao
View Article