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  • Featured Product
  • KD/KO Validated

AMPK Alpha 1 Monoclonal antibody, PBS Only

AMPK Alpha 1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1H8E1

Cat no : 66536-1-PBS

Print datasheet

Synonyms

AMPK, AMPK alpha, AMPK Alpha 1, AMPK subunit alpha 1, AMPK1, AMPKa1, PRKAA1

验证数据展示

  • WB analysis of HEK-293 using 66536-1-Ig (same clone as 66536-1-PBS)

    WB analysis of HEK-293 using 66536-1-Ig (same clone as 66536-1-PBS)

    WB result of AMPK alpha antibody (66536-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-AMPK alpha transfected HEK-293 cells. This data was developed using the same antibody clone with 66536-1-PBS in a different storage buffer formulation.

  • WB analysis using 66536-1-Ig (same clone as 66536-1-PBS)

    WB analysis using 66536-1-Ig (same clone as 66536-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66536-1-Ig (AMPK alpha antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66536-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66536-1-Ig (same clone as 66536-1-PBS)

    IHC staining of human breast cancer using 66536-1-Ig (same clone as 66536-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66536-1-Ig (AMPK alpha antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66536-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRKAA1 antibody included
  • IHC staining of human breast cancer using 66536-1-Ig (same clone as 66536-1-PBS)

    IHC staining of human breast cancer using 66536-1-Ig (same clone as 66536-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66536-1-Ig (AMPK alpha antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66536-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRKAA1 antibody included
Planning an IHC experiment? We recommend our IHCeasy PRKAA1 Ready-To-Use IHC Kit. PRKAA1 primary antibody included.

产品信息

66536-1-PBS targets AMPK Alpha 1 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Rat, Mouse samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity Human, Rat, Mouse
Immunogen AMPK Alpha 1 fusion protein Ag24744 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name protein kinase, AMP-activated, alpha 1 catalytic subunit
Synonyms AMPK, AMPK alpha, AMPK Alpha 1, AMPK subunit alpha 1, AMPK1, AMPKa1, PRKAA1
Calculated Molecular Weight 63 kDa
Observed Molecular Weight 64 kDa
GenBank Accession NumberBC012622
Gene Symbol AMPK Alpha 1
Gene ID (NCBI) 5562
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13131
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The mammalian 5-prime-AMP-activated protein kinase (AMPK) appears to play a role in protecting cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. PRKAA1 is also named as AMPK1, ACACA kinase, HMGCR kinase. It is a mammalian homologue of sucrose non-fermenting protein kinase (SNF-1), which belongs to a serine/threonine protein kinase family. It has 2 isoforms with molecular mass of 63-66 kDa produced by alternative splicing.