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AMPK Alpha Recombinant antibody

AMPK Alpha Uni-rAbTM Recombinant Antibody for WB, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

4N5

Cat no : 80209-5-RR

Print datasheet

Synonyms

AMPK Alpha 1, PRKAA1, AMPK, Acetyl-CoA carboxylase kinase, ACACA kinase

验证数据展示

  • WB analysis using 80209-5-RR

    WB analysis using 80209-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80209-5-RR (AMPK alpha antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of rat brain using 80209-5-RR

    IF Staining of rat brain using 80209-5-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using AMPK Alpha antibody (80209-5-RR, Clone: 4N5 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 80209-5-RR

    Affinity and Kinetic Characterization of 80209-5-RR

    Biolayer interferometry (BLl) kinetic assays of 80209-5-RR against Human AMPK alpha were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inHepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IF-P detected inrat brain tissue
Planning an IHC experiment? We recommend our IHCeasy PRKAA1 Ready-To-Use IHC Kit. PRKAA1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

80209-5-RR targets AMPK Alpha in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF-P, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen AMPK Alpha fusion protein Ag1367 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name protein kinase, AMP-activated, alpha 1 catalytic subunit
Synonyms AMPK Alpha 1, PRKAA1, AMPK, Acetyl-CoA carboxylase kinase, ACACA kinase
Calculated Molecular Weight 63 kDa
Observed Molecular Weight 64 kDa
GenBank Accession NumberBC012622
Gene Symbol AMPK Alpha 1
Gene ID (NCBI) 5562
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13131
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The mammalian 5-prime-AMP-activated protein kinase (AMPK) appears to play a role in protecting cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. PRKAA1 is also named as AMPK1, ACACA kinase, HMGCR kinase. It is a mammalian homologue of sucrose non-fermenting protein kinase (SNF-1), which belongs to a serine/threonine protein kinase family. It has 2 isoforms with molecular mass of 63-66 kDa produced by alternative splicing. 80209-5-RR can recognize both AMPK Alpha 1 and AMPK Alpha 2.

实验方案

Product Specific Protocols
WB protocol for AMPK Alpha antibody 80209-5-RRDownload protocol
IF protocol for AMPK Alpha antibody 80209-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols