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验证数据展示

  • WB analysis using 10633-1-AP

    WB analysis using 10633-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10633-1-AP (Aminomethyltransferase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 10633-1-AP

    IHC staining of mouse liver using 10633-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10633-1-AP (Aminomethyltransferase antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Aminomethyltransferase Polyclonal antibody

Aminomethyltransferase Polyclonal Antibody for IHC, ELISA
Cat No. 10633-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, ELISA

别名

Aminomethyltransferase, AMT, GCE, GCST, GCVT, NKH

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse kidney tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10633-1-AP targets Aminomethyltransferase in IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, ELISA Application Description
经测试反应性 human, mouse
文献引用反应性mouse
免疫原 Aminomethyltransferase fusion protein Ag0990 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 aminomethyltransferase
别名 Aminomethyltransferase, AMT, GCE, GCST, GCVT, NKH
计算分子量 44 kDa
观测分子量40 kDa
GenBank蛋白编号BC007546
基因名称 AMT
Gene ID (NCBI) 275
RRIDAB_2305496
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP48728
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Aminomethyltransferase antibody 10633-1-APDownload protocol
IHC protocol for Aminomethyltransferase antibody 10633-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

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mouse

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mouse

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Authors - Jennifer Blaze
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