Amylase Alpha Polyclonal antibody

Amylase Alpha Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 12540-1-AP

Print datasheet

Synonyms

Alpha amylase 2B, AMY2, AMY2B, Amylase Alpha, Amylase, alpha, amylase, alpha 2B (pancreatic), Carcinoid alpha amylase



经过测试的应用

Positive WB detected inmouse pancreas tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12540-1-AP targets Amylase Alpha in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen Amylase Alpha fusion protein Ag3260 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name amylase, alpha 2B (pancreatic)
Synonyms Alpha amylase 2B, AMY2, AMY2B, Amylase Alpha, Amylase, alpha, amylase, alpha 2B (pancreatic), Carcinoid alpha amylase
Calculated Molecular Weight 511 aa, 58 kDa
Observed Molecular Weight 58 kDa
GenBank Accession NumberBC011179
Gene Symbol AMY2B
Gene ID (NCBI) 280
RRIDAB_2273990
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP19961
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AMY2B(alpha-amylase 2B) belongs to the glycosyl hydrolase 13 family. This protein is involved in the endohydrolysis of 1,4-alpha-glucosidic linkages in oligosaccharides and polysaccharides. The full length 58 kDa protein has a signal peptide with 15 amino acids. Human amylases (Amy1, Amy2A, and Amy2B) commonly have two potential N-glycosylation sites (N427 and N476) in their C-terminal region (S Takashima, J Amano, 2012). 12540-1-AP can recognize both AMY2A and AMY2B.

实验方案

Product Specific Protocols
WB protocol for Amylase Alpha antibody 12540-1-APDownload protocol
IHC protocol for Amylase Alpha antibody 12540-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

ACS Nano

Inflammation and Acinar Cell Dual-Targeting Nanomedicines for Synergistic Treatment of Acute Pancreatitis via Ca2+ Homeostasis Regulation and Pancreas Autodigestion Inhibition

Authors - Yanan Wang
mouseIF

Cancer Lett

SULF2 enhances GDF15-SMAD axis to facilitate the initiation and progression of pancreatic cancer.

Authors - Ruizhe He

Biochim Biophys Acta Mol Basis Dis

Pyrazole derivative Z10 ameliorates acute pancreatitis by inhibiting the ERK/Ddt pathway

Authors - Wenying Zeng
mouseWB,IHC

Int Immunopharmacol

Gastrodin ameliorates acute pancreatitis by modulating macrophage inflammation cascade via inhibition the p38/NF-κB pathway

Authors - Yalan Jiang
mouseIHC

Mol Biol Cell

Vacuolization of mucolipidosis type II mouse exocrine gland cells represents accumulation of autolysosomes.

Authors - Boonen Marielle M
mouseIF

Biomed Pharmacother

Serotonin-RhoA/ROCK axis promotes acinar-to-ductal metaplasia in caerulein-induced chronic pancreatitis.

Authors - Xufeng Tao