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验证数据展示

  • WB analysis of mouse liver using 24484-1-AP

    WB analysis of mouse liver using 24484-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 24484-1-AP (AMZ1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 24484-1-AP

    IHC staining of human heart using 24484-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 24484-1-AP (AMZ1 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human heart using 24484-1-AP

    IHC staining of human heart using 24484-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 24484-1-AP (AMZ1 Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 24484-1-AP

    IF Staining of HepG2 using 24484-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using AMZ1 antibody (24484-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

AMZ1 Polyclonal antibody

AMZ1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 24484-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

KIAA1950, EC:3.4.-.-, Archeobacterial metalloproteinase-like protein 1, Archaemetzincin-1, Archaemetzincin 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse liver tissue, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24484-1-AP targets AMZ1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 AMZ1 fusion protein Ag20248 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 archaelysin family metallopeptidase 1
别名 KIAA1950, EC:3.4.-.-, Archeobacterial metalloproteinase-like protein 1, Archaemetzincin-1, Archaemetzincin 1
计算分子量 498 aa, 55 kDa
观测分子量 33 kDa, 28 kDa
GenBank蛋白编号BC146753
基因名称 AMZ1
Gene ID (NCBI) 155185
RRIDAB_2879565
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ400G9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AMZ1, also named as KIAA1950, is a new human Zinc metalloproteases. It exhibits aminopeptidase activity against neurogranin in vitro. AMZ1 does not hydrolyze angiotensin-2. It is mainly detected in liver and heart. AMZ1 has some isoforms with MW 55 kDa, 33 kDa, 30 kDa and 28 kDa.

实验方案

Product Specific Protocols
WB protocol for AMZ1 antibody 24484-1-APDownload protocol
IHC protocol for AMZ1 antibody 24484-1-APDownload protocol
IF protocol for AMZ1 antibody 24484-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols