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AMZ1 Polyclonal antibody

AMZ1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24484-1-AP

Print datasheet

Synonyms

KIAA1950, EC:3.4.-.-, Archeobacterial metalloproteinase-like protein 1, Archaemetzincin-1, Archaemetzincin 1

验证数据展示

  • WB analysis of mouse liver using 24484-1-AP

    WB analysis of mouse liver using 24484-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 24484-1-AP (AMZ1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 24484-1-AP

    IHC staining of human heart using 24484-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 24484-1-AP (AMZ1 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human heart using 24484-1-AP

    IHC staining of human heart using 24484-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 24484-1-AP (AMZ1 Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 24484-1-AP

    IF Staining of HepG2 using 24484-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using AMZ1 antibody (24484-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive WB detected inmouse liver tissue, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24484-1-AP targets AMZ1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen AMZ1 fusion protein Ag20248 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name archaelysin family metallopeptidase 1
Synonyms KIAA1950, EC:3.4.-.-, Archeobacterial metalloproteinase-like protein 1, Archaemetzincin-1, Archaemetzincin 1
Calculated Molecular Weight 498 aa, 55 kDa
Observed Molecular Weight 33 kDa, 28 kDa
GenBank Accession NumberBC146753
Gene Symbol AMZ1
Gene ID (NCBI) 155185
RRIDAB_2879565
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ400G9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AMZ1, also named as KIAA1950, is a new human Zinc metalloproteases. It exhibits aminopeptidase activity against neurogranin in vitro. AMZ1 does not hydrolyze angiotensin-2. It is mainly detected in liver and heart. AMZ1 has some isoforms with MW 55 kDa, 33 kDa, 30 kDa and 28 kDa.

实验方案

Product Specific Protocols
WB protocol for AMZ1 antibody 24484-1-APDownload protocol
IHC protocol for AMZ1 antibody 24484-1-APDownload protocol
IF protocol for AMZ1 antibody 24484-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols