APC1 Polyclonal antibody
APC1 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293 cells, HT-1080 cells, K-562 cells, mouse brain tissue, Transfected HEK-293 cells |
| Positive IHC detected in | mouse testis tissue, human cervical cancer tissue, human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
产品信息
21748-1-AP targets APC1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Immunogen | APC1 fusion protein Ag13399 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | anaphase promoting complex subunit 1 |
| Synonyms | TSG 24, Testis-specific gene 24 protein, Mitotic checkpoint regulator, Apc 1, Anaphase-promoting complex subunit 1 |
| Calculated Molecular Weight | 1944 aa, 217 kDa |
| Observed Molecular Weight | 200-210 kDa |
| GenBank Accession Number | BC104902 |
| Gene Symbol | APC1 |
| Gene ID (NCBI) | 64682 |
| RRID | AB_10733241 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H1A4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for APC1 antibody 21748-1-AP | Download protocol |
| IHC protocol for APC1 antibody 21748-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun DNA replication initiation factor RECQ4 possesses a role in antagonizing DNA replication initiation | ||
Oncotarget Screening key microRNAs for castration-resistant prostate cancer based on miRNA/mRNA functional synergistic network. | ||
