• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

ANAPC10 Polyclonal antibody

ANAPC10 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15788-1-AP

Print datasheet

Synonyms

DOC1, APC10, Anaphase-promoting complex subunit 10, ANAPC 10

验证数据展示

  • IHC staining of human heart using 15788-1-AP

    IHC staining of human heart using 15788-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15788-1-AP (ANAPC10 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human heart using 15788-1-AP

    IHC staining of human heart using 15788-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15788-1-AP (ANAPC10 Antibody) at dilution of 1:200 (under 40x lens).

  • WB analysis using 15788-1-AP

    WB analysis using 15788-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15788-1-AP (ANAPC10 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15788-1-AP targets ANAPC10 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen ANAPC10 fusion protein Ag8487 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name anaphase promoting complex subunit 10
Synonyms DOC1, APC10, Anaphase-promoting complex subunit 10, ANAPC 10
Calculated Molecular Weight 185 aa, 21 kDa
Observed Molecular Weight20 kDa
GenBank Accession NumberBC005217
Gene Symbol ANAPC10
Gene ID (NCBI) 10393
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UM13
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Anaphase-promoting complex subunit 10 (Anapc10) is a component of the promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase.Anapc10 recognizes ubiquitination-mediated degradation of substrates, and is expressed predominantly in the cytoplasm of spermatogonial and leptoblast cells.

实验方案

Product Specific Protocols
WB protocol for ANAPC10 antibody 15788-1-APDownload protocol
IHC protocol for ANAPC10 antibody 15788-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols