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验证数据展示

  • IHC staining of human heart using 15788-1-AP

    IHC staining of human heart using 15788-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15788-1-AP (ANAPC10 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human heart using 15788-1-AP

    IHC staining of human heart using 15788-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15788-1-AP (ANAPC10 Antibody) at dilution of 1:200 (under 40x lens).

  • WB analysis using 15788-1-AP

    WB analysis using 15788-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15788-1-AP (ANAPC10 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

ANAPC10 Polyclonal antibody

ANAPC10 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15788-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

DOC1, APC10, Anaphase-promoting complex subunit 10, ANAPC 10

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15788-1-AP targets ANAPC10 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ANAPC10 fusion protein Ag8487 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 anaphase promoting complex subunit 10
别名 DOC1, APC10, Anaphase-promoting complex subunit 10, ANAPC 10
计算分子量 185 aa, 21 kDa
观测分子量20 kDa
GenBank蛋白编号BC005217
基因名称 ANAPC10
Gene ID (NCBI) 10393
RRIDAB_3669220
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UM13
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Anaphase-promoting complex subunit 10 (Anapc10) is a component of the promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase.Anapc10 recognizes ubiquitination-mediated degradation of substrates, and is expressed predominantly in the cytoplasm of spermatogonial and leptoblast cells.

实验方案

Product Specific Protocols
WB protocol for ANAPC10 antibody 15788-1-APDownload protocol
IHC protocol for ANAPC10 antibody 15788-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols