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ANAPC10 Recombinant antibody

ANAPC10 Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240492E7

Cat No : 83570-4-RR

Print datasheet

Synonyms

DOC1, APC10, Anaphase-promoting complex subunit 10, ANAPC 10, 240492E7

验证数据展示

  • IF Staining of HeLa using 83570-4-RR

    IF Staining of HeLa using 83570-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ANAPC10 antibody (83570-4-RR, Clone: 240492E7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 83570-4-RR

    Affinity and Kinetic Characterization of 83570-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83570-4-RR against Human ANAPC10 were performed. The affinity constant is 0.141 nM.

经过测试的应用

Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83570-4-RR is ANAPC10 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen ANAPC10 fusion protein Ag8487 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name anaphase promoting complex subunit 10
Synonyms DOC1, APC10, Anaphase-promoting complex subunit 10, ANAPC 10, 240492E7
Calculated Molecular Weight 185 aa, 21 kDa
GenBank Accession NumberBC005217
Gene Symbol ANAPC10
Gene ID (NCBI) 10393
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9UM13
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The anaphase promoting complex/cyclosome (APC/C) is a cell cycle-regulated multimeric E3 ubiquitin ligase assembled from 13 individual subunits. The anaphase promoting complex subunit 10 (APC10) is a core subunit of APC/C that is highly conserved in humans. APC10 genetically and physically interacts with a series of subunits of the APC/C and is necessary for the ubiquitination activity of APC/C by enhancing the affinity of the APC/C for its substrate.

实验方案

Product Specific Protocols
IF protocol for ANAPC10 antibody 83570-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols