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验证数据展示

  • WB analysis using 81990-4-RR (same clone as 81990-4-PBS)

    WB analysis using 81990-4-RR (same clone as 81990-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81990-4-RR (Angiopoietin 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81990-4-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 81990-4-RR (same clone as 81990-4-PBS)

    IHC staining of mouse heart using 81990-4-RR (same clone as 81990-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 81990-4-RR (Angiopoietin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81990-4-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 81990-4-RR (same clone as 81990-4-PBS)

    FC experiment of HepG2 using 81990-4-RR (same clone as 81990-4-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Angiopoietin 1 Recombinant antibody (81990-4-RR, Clone:240764D10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 81990-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81990-4-RR

    Affinity and Kinetic Characterization of 81990-4-RR

    Biolayer interferometry (BLl) kinetic assays of 81990-4-RR against Human ANGPT1 were performed. The affinity constant is 6.37 nM.

Angiopoietin 1 Recombinant antibody, PBS Only

Angiopoietin 1 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 81990-4-PBS

产品说明书

CloneNo. 240764D10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, FC (Intra), ELISA

别名

ANGPT1, ANG-1, ANG1, ANG 1, AGPT

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

81990-4-PBS targets Angiopoietin 1 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 Angiopoietin 1 fusion protein Ag18829 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 angiopoietin 1
别名 ANGPT1, ANG-1, ANG1, ANG 1, AGPT
计算分子量 498 aa, 58 kDa
观测分子量70 kDa
GenBank蛋白编号BC152411
基因名称 Angiopoietin 1
Gene ID (NCBI) 284
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ15389
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Angiopoietin 1 is a 70-kDa secreted glycoprotein generated from vascular mural cells, pericytes, and certain other cells. Angiopoietin 1 participates in vascular differentiation through angiogenesis, which is the process of the growth and remodelling of existing vessels. Angiopoietin 1 is also involved in the maintenance and turnover of blood vessels in mature animals. Angiopoietin 1 binds to and activates the TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation, playing an important role in the regulation of angiogenesis. Overexpression of Angiopoietin 1 has been proven to occur in malignant glioblastoma, neuroblastoma, non-small cell lung cancer, and other tumors.