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验证数据展示

  • WB analysis of A375 using 11964-1-AP

    WB analysis of A375 using 11964-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 11964-1-AP (ANGPTL3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 11964-1-AP

    IHC staining of human liver using 11964-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11964-1-AP (ANGPTL3 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver using 11964-1-AP

    IHC staining of human liver using 11964-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11964-1-AP (ANGPTL3 Antibody) at dilution of 1:200 (under 40x lens).

ANGPTL3 Polyclonal antibody

ANGPTL3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11964-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IHC, ELISA

别名

ANG 5, Angiopoietin 5, angiopoietin like 3, Angiopoietin like protein 3, Angiopoietin related protein 3, ANGPT5, ANGPTL3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA375 cells, mouse liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

11964-1-AP targets ANGPTL3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human, rat
免疫原 ANGPTL3 fusion protein Ag2573 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 angiopoietin-like 3
别名 ANG 5, Angiopoietin 5, angiopoietin like 3, Angiopoietin like protein 3, Angiopoietin related protein 3, ANGPT5, ANGPTL3
计算分子量 460 aa, 54 kDa
观测分子量 54 kDa
GenBank蛋白编号BC058287
基因名称 ANGPTL3
Gene ID (NCBI) 27329
RRIDAB_874076
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y5C1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ANGPTL3 belongs to the angiopoietin-like protein family. ANGPTL3 is mainly synthesized by liver cells and is notably expressed in kidney podocytes. Accumulating evidences have revealed that ANGPTL3 plays a critical role in both biological processes, such as lipid metabolism, angiogenesis and haematopoietic function and pathological changes, including atherosclerosis, carcinogenesis, nephrotic syndrome, diabetes, liver diseases and so on. Thus, ANGPTL3 may serve as a potential biomarker in these diseases.

实验方案

Product Specific Protocols
WB protocol for ANGPTL3 antibody 11964-1-APDownload protocol
IHC protocol for ANGPTL3 antibody 11964-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,ratWB

Invest Ophthalmol Vis Sci

PMID: 30128492

Authors - Na Wang
View Article
humanWB

Cancer Sci

Screening of novel serum biomarkers for gastric cancer in coastal populations using a protein microarray

Authors - Yongdong Yi
View Article