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ANK1 Polyclonal antibody

ANK1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 23938-1-AP

Print datasheet

Synonyms

Ankyrin 1, Ankyrin-1, Ankyrin R, ANK-1, ANK 1

验证数据展示

  • WB analysis using 23938-1-AP

    WB analysis using 23938-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23938-1-AP (ANK1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse cerebellum using 23938-1-AP

    IHC staining of mouse cerebellum using 23938-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 23938-1-AP (ANK1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ANK1 antibody included
  • IHC staining of mouse cerebellum using 23938-1-AP

    IHC staining of mouse cerebellum using 23938-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 23938-1-AP (ANK1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ANK1 antibody included
  • IHC staining of rat cerebellum using 23938-1-AP

    IHC staining of rat cerebellum using 23938-1-AP

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 23938-1-AP (ANK1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ANK1 antibody included
  • IHC staining of rat cerebellum using 23938-1-AP

    IHC staining of rat cerebellum using 23938-1-AP

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 23938-1-AP (ANK1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ANK1 antibody included
  • IHC staining of human lymphoma using 23938-1-AP

    IHC staining of human lymphoma using 23938-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 23938-1-AP (ANK1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ANK1 antibody included
  • IHC staining of human stomach cancer using 23938-1-AP

    IHC staining of human stomach cancer using 23938-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 23938-1-AP (ANK1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ANK1 antibody included

经过测试的应用

Positive WB detected inK-562 cells, mouse liver tissue
Positive IHC detected inhuman stomach cancer tissue, human lymphoma tissue, mouse cerebellum tissue, rat cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ANK1 Ready-To-Use IHC Kit. ANK1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23938-1-AP targets ANK1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen ANK1 fusion protein Ag21041 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ankyrin 1, erythrocytic
Synonyms Ankyrin 1, Ankyrin-1, Ankyrin R, ANK-1, ANK 1
Calculated Molecular Weight 155 aa, 18 kDa
Observed Molecular Weight 190-250 kDa
GenBank Accession NumberBC030957
Gene Symbol ANK1
Gene ID (NCBI) 286
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDP16157
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ankyrin-1 (ANK1) is also named as Ankyrin-R and Erythrocyte ankyrin. The stability and shape of the erythrocyte membrane is provided by the ankyrin-1 complex (PMID:18440852). ANK1 can attache integral membrane proteins to cytoskeletal elements. And it binds to the erythrocyte membrane protein band 4.2, to Na-K ATPase, to the lymphocyte membrane protein GP85, and to the cytoskeletal proteins fodrin, tubulin, vimentin and desmin.(PMID:12456646).

实验方案

Product Specific Protocols
WB protocol for ANK1 antibody 23938-1-APDownload protocol
IHC protocol for ANK1 antibody 23938-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols