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ANK3 Polyclonal antibody

ANK3 Polyclonal Antibody for IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 27766-1-AP

Print datasheet

Synonyms

ANK 3, ANK3, Ankyrin 3, ANKYRIN G

验证数据展示

  • IHC staining of mouse brain using 27766-1-AP

    IHC staining of mouse brain using 27766-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27766-1-AP (ANK3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 27766-1-AP

    IHC staining of mouse brain using 27766-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27766-1-AP (ANK3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 27766-1-AP

    IF Staining of mouse brain using 27766-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using ANK3 antibody (27766-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse brain using 27766-1-AP

    IF Staining of mouse brain using 27766-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using ANK3 antibody (27766-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive IHC detected inmouse brain tissue, human kidney tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27766-1-AP targets ANK3 in IHC, IF-P, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications IHC, IF-P, ELISA Application Description
Tested Reactivity Human, Mouse
Immunogen ANK3 fusion protein Ag26948 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ankyrin 3, node of Ranvier (ankyrin G)
Synonyms ANK 3, ANK3, Ankyrin 3, ANKYRIN G
Calculated Molecular Weight 480 kDa
Observed Molecular Weight 204 kDa
GenBank Accession NumberNM_001149
Gene Symbol ANK3
Gene ID (NCBI) 288
RRIDAB_2880966
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for ANK3 antibody 27766-1-APDownload protocol
IF protocol for ANK3 antibody 27766-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols