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验证数据展示

  • WB analysis of HeLa using 24080-1-AP

    WB analysis of HeLa using 24080-1-AP

    Hela cells were subjected to SDS PAGE followed by western blot with 24080-1-AP (ANKLE1 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 24080-1-AP

    IHC staining of human breast cancer using 24080-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24080-1-AP (ANKLE1 Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HeLa using 24080-1-AP

    IF Staining of HeLa using 24080-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 24080-1-AP (ANKLE1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

ANKLE1 Polyclonal antibody

ANKLE1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 24080-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

ANKLE1, ANKRD41, LEM3, LEMD6

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHela cells, K-562 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24080-1-AP targets ANKLE1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human
文献引用反应性human
免疫原 ANKLE1 fusion protein Ag21253 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ankyrin repeat and LEM domain containing 1
别名 ANKLE1, ANKRD41, LEM3, LEMD6
计算分子量 615 aa, 67 kDa
观测分子量 67 kDa
GenBank蛋白编号BC125274
基因名称 ANKLE1
Gene ID (NCBI) 126549
RRIDAB_2879428
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ8NAG6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ANKLE1 antibody 24080-1-APDownload protocol
IHC protocol for ANKLE1 antibody 24080-1-APDownload protocol
IF protocol for ANKLE1 antibody 24080-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

World J Surg Oncol

Collective analysis of the expression and prognosis for LEM-domain proteins in prostate cancer.

Authors - Tianzhen He
View Article