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ANKLE2 Polyclonal antibody

ANKLE2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26293-1-AP

Print datasheet

Synonyms

LEMD7, LEM4, LEM domain-containing protein 4, KIAA0692, Ankyrin repeat and LEM domain-containing protein 2

验证数据展示

  • WB analysis of HeLa using 26293-1-AP

    WB analysis of HeLa using 26293-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 26293-1-AP (ANKLE2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 26293-1-AP

    IHC staining of human ovary cancer using 26293-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 26293-1-AP (ANKLE2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary cancer using 26293-1-AP

    IHC staining of human ovary cancer using 26293-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 26293-1-AP (ANKLE2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26293-1-AP targets ANKLE2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen ANKLE2 fusion protein Ag23387 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ankyrin repeat and LEM domain containing 2
Synonyms LEMD7, LEM4, LEM domain-containing protein 4, KIAA0692, Ankyrin repeat and LEM domain-containing protein 2
Observed Molecular Weight 105-110 kDa
GenBank Accession NumberBC043157
Gene Symbol ANKLE2
Gene ID (NCBI) 23141
RRIDAB_2880466
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86XL3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ANKLE2 antibody 26293-1-APDownload protocol
IHC protocol for ANKLE2 antibody 26293-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols