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ANKRD16 Polyclonal antibody

ANKRD16 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24334-1-AP

Print datasheet

Synonyms

Ankyrin repeat domain-containing protein 16, ankyrin repeat domain 16

验证数据展示

  • IF Staining of HeLa using 24334-1-AP

    IF Staining of HeLa using 24334-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ANKRD16 antibody (24334-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • WB analysis of HeLa using 24334-1-AP

    WB analysis of HeLa using 24334-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24334-1-AP (ANKRD16 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 24334-1-AP

    IHC staining of human ovary cancer using 24334-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 24334-1-AP (ANKRD16 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells, mouse liver tissue, PC-3 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24334-1-AP targets ANKRD16 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen ANKRD16 fusion protein Ag19369 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ankyrin repeat domain 16
Synonyms Ankyrin repeat domain-containing protein 16, ankyrin repeat domain 16
Calculated Molecular Weight 361 aa, 39 kDa
Observed Molecular Weight 39-45 kDa
GenBank Accession NumberBC062346
Gene Symbol ANKRD16
Gene ID (NCBI) 54522
RRIDAB_2879499
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6P6B7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ANKRD16, a vertebrate-specific protein that contains ankyrin repeats, binds directly to the catalytic domain of AlaRS. Serine that is misactivated by AlaRS is captured by the lysine side chains of ANKRD16, which prevents the charging of serine adenylates to tRNAAla and precludes serine misincorporation in nascent peptides. The deletion of Ankrd16 in the brains of Aarssti/sti mice causes widespread protein aggregation and neuron loss (PMID: 29769718).

实验方案

Product Specific Protocols
WB protocol for ANKRD16 antibody 24334-1-APDownload protocol
IHC protocol for ANKRD16 antibody 24334-1-APDownload protocol
IF protocol for ANKRD16 antibody 24334-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols