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验证数据展示

  • WB analysis of mouse skeletal muscle using 11821-1-AP (same clone as 11821-1-PBS)

    WB analysis of mouse skeletal muscle using 11821-1-AP (same clone as 11821-1-PBS)

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11821-1-AP (ANKRD2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11821-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 11821-1-AP (same clone as 11821-1-PBS)

    IHC staining of human kidney using 11821-1-AP (same clone as 11821-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 11821-1-AP (ANKRD2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 11821-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ANKRD2 antibody included
  • IHC staining of human kidney using 11821-1-AP (same clone as 11821-1-PBS)

    IHC staining of human kidney using 11821-1-AP (same clone as 11821-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 11821-1-AP (ANKRD2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 11821-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ANKRD2 antibody included
  • IP experiment of mouse skeletal muscle using 11821-1-AP (same clone as 11821-1-PBS)

    IP experiment of mouse skeletal muscle using 11821-1-AP (same clone as 11821-1-PBS)

    IP result of anti-ANKRD2 (IP:11821-1-AP, 4ug; Detection:11821-1-AP 1:500) with mouse skeletal muscle tissue lysate 3600ug. This data was developed using the same antibody clone with 11821-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 11821-1-AP (same clone as 11821-1-PBS)

    IF Staining of HeLa using 11821-1-AP (same clone as 11821-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ANKRD2 antibody (11821-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 11821-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

ANKRD2 Polyclonal antibody, PBS Only

ANKRD2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 11821-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

hArpp, ARPP, Ankyrin repeat domain-containing protein 2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy ANKRD2 Ready-To-Use IHC Kit. ANKRD2 primary antibody included.

产品信息

11821-1-PBS targets ANKRD2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ANKRD2 fusion protein Ag2389 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ankyrin repeat domain 2 (stretch responsive muscle)
别名 hArpp, ARPP, Ankyrin repeat domain-containing protein 2
计算分子量 37 aa, 2 kDa
观测分子量 42 kDa
GenBank蛋白编号BC020817
基因名称 ANKRD2
Gene ID (NCBI) 26287
RRIDAB_2057858
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9GZV1
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ANKRD2 belongs to the conserved muscle ankyrin repeat protein (MARP) family. Expression of MARPs is induced in response to physiologic stress, injury, and hypertrophy. Together with Ankrd1/CARP and DARP, ANKRD2 are members of the MARP mechanosensing proteins that form a complex with titin (N2A)/calpain 3 protease/myopalladin. Ankrd2 is thought to have dual, structural and signaling roles, and could link the elastic I-band region as a stress sensor for transcriptional control in the nucleus