• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

ANKRD53 Polyclonal antibody

ANKRD53 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24283-1-AP

Print datasheet

Synonyms

Ankyrin repeat domain-containing protein 53, ankyrin repeat domain 53

验证数据展示

  • WB analysis of HeLa using 24283-1-AP

    WB analysis of HeLa using 24283-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24283-1-AP (ANKRD53 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 24283-1-AP

    IHC staining of human kidney using 24283-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24283-1-AP (ANKRD53 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 24283-1-AP

    IHC staining of human kidney using 24283-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24283-1-AP (ANKRD53 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 24283-1-AP

    IF Staining of HepG2 using 24283-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 24283-1-AP (ANKRD53 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells
Positive IHC detected inhuman kidney tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24283-1-AP targets ANKRD53 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen ANKRD53 fusion protein Ag15030 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ankyrin repeat domain 53
Synonyms Ankyrin repeat domain-containing protein 53, ankyrin repeat domain 53
Calculated Molecular Weight 530 aa, 60 kDa
Observed Molecular Weight 60-70 kDa
GenBank Accession NumberBC035234
Gene Symbol ANKRD53
Gene ID (NCBI) 79998
RRIDAB_2879470
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N9V6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ANKRD53 also termed as ankyrin repeat domain 53 is a 533 amino-acid protein,which contains three ANK repeats and belongs to ankyrin family. The function of ANKRD53 is still non-known and need to be further studied. Ankyrins are membrane adaptor molecules that play important roles in coupling integral membrane proteins to the spectrin-based cytoskeleton network.

实验方案

Product Specific Protocols
WB protocol for ANKRD53 antibody 24283-1-APDownload protocol
IHC protocol for ANKRD53 antibody 24283-1-APDownload protocol
IF protocol for ANKRD53 antibody 24283-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols