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ANKRD54 Polyclonal antibody

ANKRD54 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25738-1-AP

Print datasheet

Synonyms

ANKRD54, ankyrin repeat domain 54

验证数据展示

  • WB analysis of mouse testis using 25738-1-AP

    WB analysis of mouse testis using 25738-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 25738-1-AP (ANKRD54 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 25738-1-AP

    IHC staining of mouse testis using 25738-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25738-1-AP (ANKRD54 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 25738-1-AP

    IHC staining of mouse testis using 25738-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25738-1-AP (ANKRD54 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse testis tissue, K-562 cells, PC-3 cells
Positive IHC detected inmouse testis tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25738-1-AP targets ANKRD54 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen ANKRD54 fusion protein Ag22759 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ankyrin repeat domain 54
Synonyms ANKRD54, ankyrin repeat domain 54
Calculated Molecular Weight 300 aa, 33 kDa
Observed Molecular Weight 33-35 kDa
GenBank Accession NumberBC066909
Gene Symbol ANKRD54
Gene ID (NCBI) 129138
RRIDAB_2880218
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6NXT1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ANKRD54 antibody 25738-1-APDownload protocol
IHC protocol for ANKRD54 antibody 25738-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols