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ANKRD57 Polyclonal antibody

ANKRD57 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24033-1-AP

Print datasheet

Synonyms

ANKRD57, ankyrin repeat domain 57, C2orf26

验证数据展示

  • WB analysis using 24033-1-AP

    WB analysis using 24033-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24033-1-AP (ANKRD57 antibody) at dilution of 1:60000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 24033-1-AP

    IP experiment of HeLa using 24033-1-AP

    IP result of anti-ANKRD57 (IP:24033-1-AP, 3ug; Detection:24033-1-AP 1:1000) with HeLa cells lysate 3000ug.

  • IHC staining of human cerebellum using 24033-1-AP

    IHC staining of human cerebellum using 24033-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 24033-1-AP (ANKRD57 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human cerebellum using 24033-1-AP

    IHC staining of human cerebellum using 24033-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 24033-1-AP (ANKRD57 Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inA431 cells, HeLa cells, HepG2 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman cerebellum tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

24033-1-AP targets ANKRD57 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen ANKRD57 fusion protein Ag21238 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ankyrin repeat domain 57
Synonyms ANKRD57, ankyrin repeat domain 57, C2orf26
Calculated Molecular Weight 525 aa, 56 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberBC036652
Gene Symbol ANKRD57
Gene ID (NCBI) 65124
RRIDAB_2879412
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ53LP3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ANKRD57 antibody 24033-1-APDownload protocol
IHC protocol for ANKRD57 antibody 24033-1-APDownload protocol
IP protocol for ANKRD57 antibody 24033-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Front Immunol

A DNA-Methylation-Driven Genes Based Prognostic Signature Reveals Immune Microenvironment in Pancreatic Cancer.

Authors - Mingjia Xiao
View Article