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ANKS1B Polyclonal antibody

ANKS1B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 24783-1-AP

Print datasheet

Synonyms

AIDA, AIDA 1, ANKS1B, ANKS2, cajalin 2, E2A PBX1 associated protein, EB 1, EB1

验证数据展示

  • IHC staining of mouse brain using 24783-1-AP

    IHC staining of mouse brain using 24783-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24783-1-AP (ANKS1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of A549 using 24783-1-AP

    WB analysis of A549 using 24783-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 24783-1-AP (ANKS1B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 24783-1-AP

    IP experiment of HeLa using 24783-1-AP

    IP result of anti-ANKS1B (IP:24783-1-AP, 4ug; Detection:24783-1-AP 1:1000) with HeLa cells lysate 3200ug.

  • IF Staining of HepG2 using 24783-1-AP

    IF Staining of HepG2 using 24783-1-AP

    Immunofluorescent analysis of HepG2 cells using 24783-1-AP (ANKS1B antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

经过测试的应用

Positive WB detected inA549 cells, HeLa cells, mouse testis tissue
Positive IP detected inHeLa cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

The immunogen of 24783-1-AP is ANKS1B Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen ANKS1B fusion protein Ag20454 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ankyrin repeat and sterile alpha motif domain containing 1B
Synonyms AIDA, AIDA 1, ANKS1B, ANKS2, cajalin 2, E2A PBX1 associated protein, EB 1, EB1
Calculated Molecular Weight 1248 aa, 138 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberBC026313
Gene Symbol ANKS1B
Gene ID (NCBI) 56899
RRIDAB_2879720
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z6G8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ANKS1B also named as AIDA or cajalin 2 is a amino acid protein, which contains 7 ANK repeats, 1 PID domain and 2 SAM domains. ANKS1B localizes in cytoplasm and is highly expressed in brain and testis. ANKS1B exists as many alternatively spliced isoforms. It interacts with AbPP to play a role in brain development. AIDA-1 also interacts with coilin in Cajal bodies to regulate pre-mRNA splicing.

实验方案

Product Specific Protocols
WB protocol for ANKS1B antibody 24783-1-APDownload protocol
IHC protocol for ANKS1B antibody 24783-1-APDownload protocol
IF protocol for ANKS1B antibody 24783-1-APDownload protocol
IP protocol for ANKS1B antibody 24783-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

EBioMedicine

Juvenile CLN3 disease is a lysosomal cholesterol storage disorder: similarities with Niemann-Pick type C disease

Authors - Jacinda Chen
View Article