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ANKS3 Polyclonal antibody

ANKS3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA and More (3)

Conjugate

Unconjugated

Cat no : 24058-1-AP

Print datasheet

Synonyms

ANKS3, KIAA1977

验证数据展示

  • WB analysis of mouse liver using 24058-1-AP

    WB analysis of mouse liver using 24058-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 24058-1-AP (ANKS3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 24058-1-AP

    IHC staining of human breast cancer using 24058-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24058-1-AP (ANKS3 Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human hepatocirrhosis using 24058-1-AP

    IHC staining of human hepatocirrhosis using 24058-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 24058-1-AP (ANKS3 Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inmouse liver tissue, human brain tissue
Positive IHC detected inhuman hepatocirrhosis tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 24058-1-AP is ANKS3 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IP, IF, CoIP
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen ANKS3 fusion protein Ag21252 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ankyrin repeat and sterile alpha motif domain containing 3
Synonyms ANKS3, KIAA1977
Calculated Molecular Weight 656 aa, 72 kDa
Observed Molecular Weight 72 kDa, 63 kDa
GenBank Accession NumberBC050387
Gene Symbol ANKS3
Gene ID (NCBI) 124401
RRIDAB_2879418
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ6ZW76
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ANKS3 (ankyrin repeat and sterile alpha motif domain containing 3) is a 656 amino acid protein that contain six ANK repeats and one SAM (sterile alpha motif) domain. Each ANK repeat consists of a 30-34 amino acid sequence that exhibits a helix-turn-helix conformation and functions to mediate protein-protein interactions. The SAM domain is a 70 amino acid domain that also functions to mediate protein-protein interactions for homo- and hetero- oligomerization. Two isoforms of ANKS3 exist due to alternative splicing events.This antibody may recognize both of them.

实验方案

Product Specific Protocols
WB protocol for ANKS3 antibody 24058-1-APDownload protocol
IHC protocol for ANKS3 antibody 24058-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IF,IP,CoIP

PLoS Biol

Bicc1 ribonucleoprotein complexes specifying organ laterality are licensed by ANKS6-induced structural remodeling of associated ANKS3

Authors - Benjamin Rothé
View Article