ANKZF1 Polyclonal antibody
ANKZF1 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | BxPC-3 cells, COLO 320 cells, HeLa cells, HepG2 cells, MCF-7 cells |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
The immunogen of 20447-1-AP is ANKZF1 Fusion Protein expressed in E. coli.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IHC, IF |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Immunogen | ANKZF1 fusion protein Ag14285 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | ankyrin repeat and zinc finger domain containing 1 |
| Synonyms | ANKZF1, Zinc finger protein 744, ZNF744 |
| Calculated Molecular Weight | 726 aa, 81 kDa |
| Observed Molecular Weight | 81 kDa |
| GenBank Accession Number | BC000238 |
| Gene Symbol | ANKZF1 |
| Gene ID (NCBI) | 55139 |
| RRID | AB_2878688 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H8Y5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ANKZF1 antibody 20447-1-AP | Download protocol |
| IHC protocol for ANKZF1 antibody 20447-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Stalled translation by mitochondrial stress upregulates a CNOT4-ZNF598 ribosomal quality control pathway important for tissue homeostasis | ||
Acta Neuropathol Commun Inefficient quality control of ribosome stalling during APP synthesis generates CAT-tailed species that precipitate hallmarks of Alzheimer's disease | ||
bioRxiv Ribosomal quality control factors inhibit repeat-associated non-AUG translation from GC-rich repeats | ||
Nucleic Acids Res Ribosomal quality control factors inhibit repeat-associated non-AUG translation from GC-rich repeats | ||
Oncol Rep Overexpression of ZNF169 promotes the growth and proliferation of colorectal cancer cells via the upregulation of ANKZF1 |
