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  • Featured Product
  • KD/KO Validated

ANLN Monoclonal antibody, PBS Only

ANLN Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1B4A8

Cat no : 66643-1-PBS

Print datasheet

Synonyms

Anillin, Scraps, scra, anillin, actin binding protein, Actin binding protein anillin

验证数据展示

  • WB analysis using 66643-1-Ig (same clone as 66643-1-PBS)

    WB analysis using 66643-1-Ig (same clone as 66643-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66643-1-Ig (ANLN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66643-1-PBS in a different storage buffer formulation.

  • WB analysis of MCF-7 using 66643-1-Ig (same clone as 66643-1-PBS)

    WB analysis of MCF-7 using 66643-1-Ig (same clone as 66643-1-PBS)

    WB result of ANLN antibody (66643-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ANLN transfected MCF-7 cells. This data was developed using the same antibody clone with 66643-1-PBS in a different storage buffer formulation.

产品信息

66643-1-PBS targets ANLN in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen ANLN fusion protein Ag19294 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name anillin, actin binding protein
Synonyms Anillin, Scraps, scra, anillin, actin binding protein, Actin binding protein anillin
Calculated Molecular Weight 1124 aa, 124 kDa
Observed Molecular Weight 110 kDa~120 kDa
GenBank Accession NumberBC034692
Gene Symbol ANLN
Gene ID (NCBI) 54443
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9NQW6
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Anillin, an actin-binding protein, is a conserved cleavage furrow component important for cytokinesis. The human anillin protein localizes to the nucleus during interphase, the cortex following nuclear envelope breakdown, and the cleavage furrow during cytokinesis. It interactions with at least two other furrow proteins, actin and the septins through its C-terminal PH domain and N-terminal actin-binding domain. In anillin knockdown cells, the cleavage furrow ingressed but failed to complete the ingression. Anillin has been found to be overexpressed in tumors and may serve as promising marker for pancreatic carcinoma molecular diagnostics as well as a prognostic indicator for non-small cell lung cancers.