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TMEM16A/DOG1 Polyclonal antibody
TMEM16A/DOG1 Polyclonal Antibody for IHC, IF-P, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
IHC, IF-P, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human lung cancer tissue |
Planning an IHC experiment? We recommend our IHCeasy TMEM16A/DOG1 Ready-To-Use IHC Kit. TMEM16A/DOG1 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12652-1-AP targets TMEM16A/DOG1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | IHC, IF-P, ELISA Application Description |
| Cited Applications | WB, IHC, IF |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Immunogen | TMEM16A/DOG1 fusion protein Ag3320 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | anoctamin 1, calcium activated chloride channel |
| Synonyms | ANO1, ORAOV2, DOG1, Discovered on gastrointestinal stromal tumors protein 1, Anoctamin-1 |
| Calculated Molecular Weight | 986 aa, 114 kDa |
| Observed Molecular Weight | 114 kDa |
| GenBank Accession Number | BC027590 |
| Gene Symbol | TMEM16A |
| Gene ID (NCBI) | 55107 |
| RRID | AB_2722560 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5XXA6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ANO1 also named as DOG1, ORAOV2, TAOS2 and TMEM16A, acts as a calcium-activated chloride channel. It is required for normal tracheal development. ANO1 (or DOG1) is used as an aid in the identification and diagnosis of gastrointestinal stromal tumors (GIST) within the context of an antibody panel, the patient's clinical history, and other diagnostic tests evaluated by a qualified pathologist. The immunogen is the C-terminal of ANO1. This antibody can recognize isoform1 and isoform2 of human ANO1.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for TMEM16A/DOG1 antibody 12652-1-AP | Download protocol |
| IF protocol for TMEM16A/DOG1 antibody 12652-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Elife Gq activity- and β-arrestin-1 scaffolding-mediated ADGRG2/CFTR coupling are required for male fertility. | ||
Biomed Pharmacother Calcium-activated chloride channel is involved in the onset of diarrhea triggered by EGFR tyrosine kinase inhibitor treatment in rats. | ||
Am J Physiol Gastrointest Liver Physiol miR-128 participates in the pathogenesis of chronic constipation by regulating the p38α/M-CSF inflammatory signaling pathway. | ||
Cell Death Dis Dual role of Ca2+-activated Cl- channel transmembrane member 16A in lipopolysaccharide-induced intestinal epithelial barrier dysfunction in vitro. | ||
Front Oncol Primary extra-gastrointestinal stromal tumor of retroperitoneum: Clinicopathologic characteristics and prognosis of six cases |
