验证数据展示
经过测试的应用
| Positive WB detected in | Y79 cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse eye tissue, human breast cancer tissue, rat eye tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | rat eye tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20647-1-AP targets ANO2 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF-P, IP, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | anoctamin 2 |
| 别名 | anoctamin 2, Anoctamin-2, C12orf3, TMEM16B, Transmembrane protein 16B |
| 计算分子量 | 114 kDa |
| 观测分子量 | 114 kDa |
| GenBank蛋白编号 | NM_020373 |
| 基因名称 | ANO2 |
| Gene ID (NCBI) | 57101 |
| RRID | AB_10696181 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NQ90 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ANO2, also named as C12orf3 and TMEM16B, belongs to the anoctamin family. It acts as a calcium-activated chloride channel (CaCC), mostly in photoreceptors. It may mediate olfactory amplification in olfactory sensory neurons (OSNs) and light perception amplification in retina.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ANO2 antibody 20647-1-AP | Download protocol |
| IHC protocol for ANO2 antibody 20647-1-AP | Download protocol |
| IF protocol for ANO2 antibody 20647-1-AP | Download protocol |
| IP protocol for ANO2 antibody 20647-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
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