验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, HEK-293 cells, HeLa cells, HEK-293T cells |
| Positive IHC detected in | human colon tissue, human colon cancer tissue, human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10843-1-AP targets ANP32B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | ANP32B fusion protein Ag1274 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | acidic (leucine-rich) nuclear phosphoprotein 32 family, member B |
| 别名 | Silver-stainable protein SSP29, Putative HLA-DR-associated protein I-2, PHAPI2, APRIL, Acidic leucine-rich nuclear phosphoprotein 32 family member B |
| 计算分子量 | 29 kDa |
| 观测分子量 | 29 kDa |
| GenBank蛋白编号 | BC013003 |
| 基因名称 | ANP32B |
| Gene ID (NCBI) | 10541 |
| RRID | AB_2056460 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q92688 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ANP32B is a member of the highly conserved acidic leucine-rich nuclear phosphoprotein 32 (ANP32) family. The ANP32 proteins have been implicated in a broad array of physiological processes, including cell differentiation, apoptotic cell death, and cell proliferation. ANP32B was identified as a novel direct substrate for caspase-3 and acted as a negative regulator for leukemic cell apoptosis. ANP32B controls the expression of the dendritic cell maturation factor CD83 by regulating the transport of its mRNA to the cytoplasm. It also modulates the activity of the transcription factor Kruppel-like factor 5 (KLF5).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ANP32B antibody 10843-1-AP | Download protocol |
| IHC protocol for ANP32B antibody 10843-1-AP | Download protocol |
| IF protocol for ANP32B antibody 10843-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Adv The SUMO-interacting motif in NS2 promotes adaptation of avian influenza virus to mammals | ||
Proc Natl Acad Sci U S A Acidic nuclear phosphoprotein 32kDa (ANP32)B-deficient mouse reveals a hierarchy of ANP32 importance in mammalian development. | ||
Carcinogenesis Downregulation of ANP32B, a novel substrate of caspase-3, enhances caspase-3 activation and apoptosis induction in myeloid leukemic cells.
| ||
Cell Death Dis ANP32B deficiency impairs proliferation and suppresses tumor progression by regulating AKT phosphorylation.
| ||
J Virol ANP32 proteins are essential for influenza virus replication in human cells.
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