验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, PC-3 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human ovary tissue, human kidney tissue, human placenta tissue, human testis tissue, human lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16723-1-AP targets ANTXR2 in WB, IP, IHC, CoIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IP, IHC, CoIP |
| 经测试反应性 | human |
| 文献引用反应性 | human, Camelus bactrianus |
| 免疫原 | ANTXR2 fusion protein Ag10162 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | anthrax toxin receptor 2 |
| 别名 | anthrax toxin receptor 2, ANTXR2, CMG 2, CMG2, ISH, JHF |
| 计算分子量 | 54 kDa |
| 观测分子量 | 55 kDa |
| GenBank蛋白编号 | BC107876 |
| 基因名称 | ANTXR2 |
| Gene ID (NCBI) | 118429 |
| RRID | AB_2056741 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P58335 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ANTXR2 (anthrax toxin receptor 2), also known as CMG2 (capillary morphogenesis gene 2 protein) is a transmembrane protein that is induced during capillary morphogenesis. It contains a vWA domain that shows strong binding to laminin and collagen IV, suggesting that this protein plays a role in basement membrane matrix assembly and endothelial cell morphogenesis (PMID: 11683410; 14508707). Like its paralog TEM8 (ANTXR1), ANTXR2 also functions as a receptor for anthrax toxin. Mutations in ANTXR2 result in the allelic disorders juvenile hyaline fibromatosis and infantile systemic hyalinosis (PMID: 14508707; 12973667; 22383261).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ANTXR2 antibody 16723-1-AP | Download protocol |
| IHC protocol for ANTXR2 antibody 16723-1-AP | Download protocol |
| IP protocol for ANTXR2 antibody 16723-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Angiogenesis Capillary morphogenesis gene 2 (CMG2) mediates growth factor-induced angiogenesis by regulating endothelial cell chemotaxis.
| ||
Oncogene Capillary morphogenesis gene 2 maintains gastric cancer stem-like cell phenotype by activating a Wnt/β-catenin pathway. | ||
J Pathol Capillary morphogenesis protein 2 is a novel prognostic biomarker and plays oncogenic roles in glioma. | ||
FASEB J Genetic deletion of CMG2 exacerbates systemic-to-pulmonary shunt-induced pulmonary arterial hypertension. | ||
Reprod Domest Anim Screening for reproductive biomarkers in Bactrian camel via iTRAQ analysis of proteomes. | ||
Proc Natl Acad Sci U S A Anthrax toxin requires ZDHHC5-mediated palmitoylation of its surface-processing host enzymes. |
