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验证数据展示

  • WB analysis using 17478-1-AP

    WB analysis using 17478-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17478-1-AP (ANUBL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 17478-1-AP

    IHC staining of human stomach cancer using 17478-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17478-1-AP (ANUBL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ANUBL1 Polyclonal antibody

ANUBL1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 17478-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

AN1 type zinc finger protein 4, ANUBL1, ZFAND4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells, mouse kidney tissue, mouse liver tissue, rat kidney tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17478-1-AP targets ANUBL1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ANUBL1 fusion protein Ag11505 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 AN1, ubiquitin-like, homolog (Xenopus laevis)
别名 AN1 type zinc finger protein 4, ANUBL1, ZFAND4
计算分子量 653 aa, 72 kDa
观测分子量70-80 kDa
GenBank蛋白编号BC048968
基因名称 ANUBL1
Gene ID (NCBI) 93550
RRIDAB_2923568
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86XD8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ANUBL1 is also named as ZFAND4. The expression of ANUBL1 was specifically upregulated in gastric cancer and it was positively associated with grades of gastric cancer. Moreover, it promoted proliferation of gastric cancer SGC-7901 cells. The mechanism of ANUBL1 upregulation in gastric cancer is that miR-182 suppressed the expression of ANUBL1 through targeting its 3'UTR, and ANUBL1 could downregulate the expression of anti-proliferation-associated miRNAs (miR-148b, miR-375 and miR-182) in SGC-7901 cells. The calculated molecular weight of ANUBL1 is 80 kDa (PMID: 25682742).

实验方案

Product Specific Protocols
WB protocol for ANUBL1 antibody 17478-1-APDownload protocol
IHC protocol for ANUBL1 antibody 17478-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols