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  • Featured Product
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Annexin A1 Polyclonal antibody

Annexin A1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 21990-1-AP

Print datasheet

Synonyms

ANXA1, ANX1, Annexin-1, Annexin I, Annexin Ac2-26

验证数据展示

  • WB analysis using 21990-1-AP

    WB analysis using 21990-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21990-1-AP (Annexin A1 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 21990-1-AP

    WB analysis of HeLa using 21990-1-AP

    WB result of Annexin A1 antibody (21990-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Annexin A1 transfected HeLa cells.

  • IHC staining of human oesophagus using 21990-1-AP

    IHC staining of human oesophagus using 21990-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 21990-1-AP (Annexin A1 antibody at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with ANXA1 antibody included
  • IHC staining of human oesophagus using 21990-1-AP

    IHC staining of human oesophagus using 21990-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 21990-1-AP (Annexin A1 antibody at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with ANXA1 antibody included
  • IHC staining of human colon using 21990-1-AP

    IHC staining of human colon using 21990-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 21990-1-AP (Annexin A1 antibody) at dilution of 1:5000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ANXA1 antibody included
  • IHC staining of human colon using 21990-1-AP

    IHC staining of human colon using 21990-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 21990-1-AP (Annexin A1 antibody) at dilution of 1:5000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ANXA1 antibody included
  • FC experiment of NIH/3T3 using 21990-1-AP

    FC experiment of NIH/3T3 using 21990-1-AP

    1X10^6 NIH/3T3 cells were intracellularly stained with 0.5 ug Anti-Human Annexin A1 (21990-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IP experiment of HeLa using 21990-1-AP

    IP experiment of HeLa using 21990-1-AP

    IP result of anti-Annexin A1 (IP:21990-1-AP, 4ug; Detection:21990-1-AP 1:20000) with HeLa cells lysate 1120 ug.

  • IF Staining of A549 using 21990-1-AP

    IF Staining of A549 using 21990-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using Annexin A1 antibody (21990-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive WB detected inA431 cells, multi-cells, MDA-MB-453s cells, HeLa cells, U-937 cells, HepG2 cells, MCF-7 cells, C6 cells, A549 cells, K-562 cells, NIH/3T3 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon tissue, human kidney tissue, human lung tissue, human oesophagus tissue, human ovary tumor tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells
Positive FC (Intra) detected inNIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy ANXA1 Ready-To-Use IHC Kit. ANXA1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21990-1-AP targets Annexin A1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, bovine
Immunogen Annexin A1 fusion protein Ag17273 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name annexin A1
Synonyms ANXA1, ANX1, Annexin-1, Annexin I, Annexin Ac2-26
Calculated Molecular Weight 346 aa, 39 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC001275
Gene Symbol Annexin A1
Gene ID (NCBI) 301
RRIDAB_11182596
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP04083
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ANXA1, also named as ANX1, LPC1 and p35, belongs to the annexin family. It is a member of the annexin family of Ca2+-binding and phospholipidbinding proteins, and it is particularly abundant in various populations of peripheral blood leukocytes. ANXA1 regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity. ANXA1 is a pleiotrophic protein produced by many cell types including peripheral blood leucocytes.

实验方案

Product Specific Protocols
WB protocol for Annexin A1 antibody 21990-1-APDownload protocol
IHC protocol for Annexin A1 antibody 21990-1-APDownload protocol
IF protocol for Annexin A1 antibody 21990-1-APDownload protocol
IP protocol for Annexin A1 antibody 21990-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF

Nature

Evolutionarily divergent mTOR remodels translatome for tissue regeneration

Authors - Olena Zhulyn
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IP

Cell Host Microbe

Global mapping of Salmonella enterica-host protein-protein interactions during infection.

Authors - Philipp Walch
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humanWB

Oncogene

The E3 ligase RBCK1 reduces the sensitivity of ccRCC to sunitinib through the ANKRD35-MITD1-ANXA1 axis

Authors - Yapeng Wang
  • KD Validated
View Article
humanWB,IHC,IF,IP

Elife

ER-luminal [Ca2+] regulation of InsP3 receptor gating mediated by an ER-luminal peripheral Ca2+-binding protein.

Authors - Horia Vais
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humanWB

Mol Ther

Membrane Stabilization by Modified Steroid Offers a Potential Therapy for Muscular Dystrophy Due to Dysferlin Deficit.

Authors - Sen Chandra Sreetama
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humanWB

Cancer Immunol Immunother

Circulating PD-L1 is associated with T cell infiltration and predicts prognosis in patients with CRLM following hepatic resection.

Authors - Xiuxing Chen
View Article