验证数据展示

WB analysis using 21990-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 21990-1-AP (Annexin A1 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 21990-1-AP
WB result of Annexin A1 antibody (21990-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Annexin A1 transfected HeLa cells.
WB analysis using 21990-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 21990-1-AP (Annexin A1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.
IHC staining of human oesophagus using 21990-1-AP
Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 21990-1-AP (Annexin A1 antibody at dilution of 1:200 (under 40x lens).
Ready-to-use IHC kits available with ANXA1 antibody included
IHC staining of human oesophagus using 21990-1-AP
Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 21990-1-AP (Annexin A1 antibody at dilution of 1:200 (under 10x lens).
Ready-to-use IHC kits available with ANXA1 antibody included
IHC staining of human colon using 21990-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 21990-1-AP (Annexin A1 antibody) at dilution of 1:5000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with ANXA1 antibody included
IHC staining of human colon using 21990-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 21990-1-AP (Annexin A1 antibody) at dilution of 1:5000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with ANXA1 antibody included
FC experiment of NIH/3T3 using 21990-1-AP
1X10^6 NIH/3T3 cells were intracellularly stained with 0.5 ug Anti-Human Annexin A1 (21990-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
FC experiment of Jurkat using 21990-1-AP
1x10^6 Jurkat cells were intracellularly stained with 0.25 ug Annexin A1 Polyclonal antibody (21990-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
IP experiment of HeLa using 21990-1-AP
IP result of anti-Annexin A1 (IP:21990-1-AP, 4ug; Detection:21990-1-AP 1:20000) with HeLa cells lysate 1120 ug.
IF Staining of A549 using 21990-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using Annexin A1 antibody (21990-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Featured Product
KD/KO Validated

Annexin A1 Polyclonal antibody

Annexin A1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 21990-1-AP

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Publications(38)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (1)

Synonyms

ANXA1, ANX1, Annexin-1, Annexin I, Annexin Ac2-26

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size:

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概述

经过测试的应用
产品信息
发表文章 (38)
实验方案

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MSDS

经过测试的应用

Positive WB detected in	A549 cells, A431 cells, C6 cells, HeLa cells, HepG2 cells, MCF-7 cells, MDA-MB-453s cells, multi-cells, U-937 cells, K-562 cells, NIH/3T3 cells, Calu-1 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human colon tissue, human kidney tissue, human lung tissue, human oesophagus tissue, human ovary tumor tissue, human small intestine tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A549 cells
Positive FC (Intra) detected in	NIH/3T3 cells, Jurkat cells

Planning an IHC experiment? We recommend our IHCeasy ANXA1 Ready-To-Use IHC Kit. ANXA1 primary antibody included.

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:2500-1:10000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 6 publications below
WB	See 30 publications below
IHC	See 7 publications below
IF	See 12 publications below
IP	See 5 publications below

产品信息

21990-1-AP targets Annexin A1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications	WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited Applications	WB, IHC, IF, IP
Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, bovine
Immunogen	Annexin A1 fusion protein Ag17273 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	annexin A1
Synonyms	ANXA1, ANX1, Annexin-1, Annexin I, Annexin Ac2-26
Calculated Molecular Weight	346 aa, 39 kDa
Observed Molecular Weight	37 kDa
GenBank Accession Number	BC001275
Gene Symbol	Annexin A1
Gene ID (NCBI)	301
RRID	AB_11182596
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P04083
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

ANXA1, also named as ANX1, LPC1 and p35, belongs to the annexin family. It is a member of the annexin family of Ca2+-binding and phospholipidbinding proteins, and it is particularly abundant in various populations of peripheral blood leukocytes. ANXA1 regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity. ANXA1 is a pleiotrophic protein produced by many cell types including peripheral blood leucocytes.

实验方案

Product Specific Protocols
WB protocol for Annexin A1 antibody 21990-1-AP	Download protocol
IHC protocol for Annexin A1 antibody 21990-1-AP	Download protocol
IF protocol for Annexin A1 antibody 21990-1-AP	Download protocol
IP protocol for Annexin A1 antibody 21990-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
mouse	WB,IF	Nature Evolutionarily divergent mTOR remodels translatome for tissue regeneration Authors - Olena Zhulyn View Article
	IP	Cell Host Microbe Global mapping of Salmonella enterica-host protein-protein interactions during infection. Authors - Philipp Walch View Article
human	WB	Oncogene The E3 ligase RBCK1 reduces the sensitivity of ccRCC to sunitinib through the ANKRD35-MITD1-ANXA1 axis Authors - Yapeng Wang KD Validated View Article
human	WB,IHC,IF,IP	Elife ER-luminal [Ca2+] regulation of InsP3 receptor gating mediated by an ER-luminal peripheral Ca2+-binding protein. Authors - Horia Vais View Article
human	WB	Mol Ther Membrane Stabilization by Modified Steroid Offers a Potential Therapy for Muscular Dystrophy Due to Dysferlin Deficit. Authors - Sen Chandra Sreetama View Article
human	WB	Cancer Immunol Immunother Circulating PD-L1 is associated with T cell infiltration and predicts prognosis in patients with CRLM following hepatic resection. Authors - Xiuxing Chen View Article

验证数据展示

WB analysis using 21990-1-AP

WB analysis of HeLa using 21990-1-AP

WB analysis using 21990-1-AP

IHC staining of human oesophagus using 21990-1-AP

IHC staining of human oesophagus using 21990-1-AP

IHC staining of human colon using 21990-1-AP

IHC staining of human colon using 21990-1-AP

FC experiment of NIH/3T3 using 21990-1-AP

FC experiment of Jurkat using 21990-1-AP

IP experiment of HeLa using 21990-1-AP

IF Staining of A549 using 21990-1-AP

Annexin A1 Polyclonal antibody

Annexin A1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

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经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Evolutionarily divergent mTOR remodels translatome for tissue regeneration

Global mapping of Salmonella enterica-host protein-protein interactions during infection.

The E3 ligase RBCK1 reduces the sensitivity of ccRCC to sunitinib through the ANKRD35-MITD1-ANXA1 axis

ER-luminal [Ca2+] regulation of InsP3 receptor gating mediated by an ER-luminal peripheral Ca2+-binding protein.

Membrane Stabilization by Modified Steroid Offers a Potential Therapy for Muscular Dystrophy Due to Dysferlin Deficit.

Circulating PD-L1 is associated with T cell infiltration and predicts prognosis in patients with CRLM following hepatic resection.