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Annexin A1 Polyclonal antibody

Annexin A1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 21990-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (1)

ANXA1, ANX1, Annexin-1, Annexin I, Annexin Ac2-26

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inA549 cells, A431 cells, C6 cells, HeLa cells, HepG2 cells, MCF-7 cells, MDA-MB-453s cells, multi-cells, U-937 cells, K-562 cells, NIH/3T3 cells, Calu-1 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon tissue, human kidney tissue, human lung tissue, human oesophagus tissue, human ovary tumor tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells
Positive FC (Intra) detected inNIH/3T3 cells, Jurkat cells
Planning an IHC experiment? We recommend our IHCeasy ANXA1 Ready-To-Use IHC Kit. ANXA1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21990-1-AP targets Annexin A1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, bovine
免疫原 Annexin A1 fusion protein Ag17273 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 annexin A1
别名 ANXA1, ANX1, Annexin-1, Annexin I, Annexin Ac2-26
计算分子量 346 aa, 39 kDa
观测分子量 37 kDa
GenBank蛋白编号BC001275
基因名称 Annexin A1
Gene ID (NCBI) 301
RRIDAB_11182596
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP04083
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ANXA1, also named as ANX1, LPC1 and p35, belongs to the annexin family. It is a member of the annexin family of Ca2+-binding and phospholipidbinding proteins, and it is particularly abundant in various populations of peripheral blood leukocytes. ANXA1 regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity. ANXA1 is a pleiotrophic protein produced by many cell types including peripheral blood leucocytes.

实验方案

Product Specific Protocols
WB protocol for Annexin A1 antibody 21990-1-APDownload protocol
IHC protocol for Annexin A1 antibody 21990-1-APDownload protocol
IF protocol for Annexin A1 antibody 21990-1-APDownload protocol
IP protocol for Annexin A1 antibody 21990-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF

Nature

Evolutionarily divergent mTOR remodels translatome for tissue regeneration

Authors - Olena Zhulyn
IP

Cell Host Microbe

Global mapping of Salmonella enterica-host protein-protein interactions during infection.

Authors - Philipp Walch
humanWB

Oncogene

The E3 ligase RBCK1 reduces the sensitivity of ccRCC to sunitinib through the ANKRD35-MITD1-ANXA1 axis

Authors - Yapeng Wang
  • KD Validated
humanWB,IHC,IF,IP

Elife

ER-luminal [Ca2+] regulation of InsP3 receptor gating mediated by an ER-luminal peripheral Ca2+-binding protein.

Authors - Horia Vais
humanWB

Mol Ther

Membrane Stabilization by Modified Steroid Offers a Potential Therapy for Muscular Dystrophy Due to Dysferlin Deficit.

Authors - Sen Chandra Sreetama
humanWB

Cancer Immunol Immunother

Circulating PD-L1 is associated with T cell infiltration and predicts prognosis in patients with CRLM following hepatic resection.

Authors - Xiuxing Chen
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