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Annexin A1 Polyclonal antibody

Annexin A1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 55018-1-AP

Print datasheet

Synonyms

ANXA1, ANX1, Annexin-1, Annexin I, Annexin Ac2-26



经过测试的应用

Positive WB detected inC2C12 cell, HeLa cells, NIH/3T3 cells, HepG2 cells, C6 cells, K-562 cells
Positive IHC detected inhuman colon tissue, human oesophagus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ANXA1 Ready-To-Use IHC Kit. ANXA1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55018-1-AP targets Annexin A1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, monkey
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name annexin A1
Synonyms ANXA1, ANX1, Annexin-1, Annexin I, Annexin Ac2-26
Calculated Molecular Weight 40 kDa
Observed Molecular Weight 39 kDa
GenBank Accession NumberNM_000700
Gene Symbol Annexin A1
Gene ID (NCBI) 301
RRIDAB_10860266
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP04083
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ANXA1, also named as ANX1, LPC1 and p35, belongs to the annexin family. It is a member of the annexin family of Ca2+-binding and phospholipidbinding proteins, and it is particularly abundant in various populations of peripheral blood leukocytes. ANXA1 regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity. ANXA1 is a pleiotrophic protein produced by many cell types including peripheral blood leucocytes. The antibody is specific to ANXA1.

实验方案

Product Specific Protocols
WB protocol for Annexin A1 antibody 55018-1-APDownload protocol
IHC protocol for Annexin A1 antibody 55018-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Expert Rev Mol Diagn

Co-overexpression of Hsp90-β and annexin A1 with a significantly positive correlation contributes to the diagnosis of lung cancer.

Authors - Rong Biaoxue
mouseIF

J Cell Sci

Platelet-derived microvesicles regulate vascular smooth muscle cell energy metabolism via PRKAA after intimal injury.

Authors - Jing Yan
monkeyWB

J Proteomics

iTRAQ-based comparative proteomic analysis of Vero cells infected with virulent and CV777 vaccine strain-like strains of porcine epidemic diarrhea virus.

Authors - Xiaozhen Guo
mouseWB,IF

Mediators Inflamm

Interaction between ANXA1 and GATA-3 in Immunosuppression of CD4(+) T Cells.

Authors - Peng Huang
humanWB, IHC

J Exp Clin Cancer Res

Upregulation of Hsp90-beta and annexin A1 correlates with poor survival and lymphatic metastasis in lung cancer patients.

Authors - Biaoxue Rong R
humanIHC

Am J Transl Res

Elevated serum annexin A1 as potential diagnostic marker for lung cancer: a retrospective case-control study.

Authors - Biaoxue Rong