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验证数据展示

  • FC experiment of PC-3 using 83929-2-RR

    FC experiment of PC-3 using 83929-2-RR

    1x10^6 PC-3 cells were intracellularly stained with 0.25 ug Anxa5 Recombinant Antibody (83929-2-RR, Clone:240621B10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HeLa using 83929-2-RR

    IF Staining of HeLa using 83929-2-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ANXA5 antibody (83929-2-RR, Clone: 240621B10 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of HeLa using 83929-2-RR

    FC experiment of HeLa using 83929-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Anxa5 Recombinant Antibody (83929-2-RR, Clone:240621B10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IHC staining of human colon using 83929-2-RR

    IHC staining of human colon using 83929-2-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 83929-2-RR (ANXA5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon using 83929-2-RR

    IHC staining of human colon using 83929-2-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 83929-2-RR (ANXA5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83929-2-RR

    Affinity and Kinetic Characterization of 83929-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83929-2-RR against Human ANXA5 were performed. The affinity constant is 18.2 nM.

ANXA5 Recombinant antibody

ANXA5 Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83929-2-RR

Print datasheet

CloneNo. 240621B10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, FC (Intra), ELISA

Synonyms

Annexin A5, Annexin-5, Annexin V, Annexin 5, Anchorin CII

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inPC-3 cells, HeLa cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83929-2-RR targets ANXA5 in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen ANXA5 fusion protein Ag0741 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name annexin A5
Synonyms Annexin A5, Annexin-5, Annexin V, Annexin 5, Anchorin CII
Calculated Molecular Weight 36 kDa
Observed Molecular Weight 32-36 kDa
GenBank Accession NumberBC001429
Gene Symbol Annexin V
Gene ID (NCBI) 308
RRIDAB_3671510
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP08758
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Annexin A5 (ANXA5), is a member of the structurally related family of annexin proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII.

实验方案

Product Specific Protocols
IHC protocol for ANXA5 antibody 83929-2-RRDownload protocol
IF protocol for ANXA5 antibody 83929-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols