验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, human brain tissue, mouse brain tissue, mouse placenta tissue, mouse kidney tissue, A549 cells, Jurkat cells |
Positive IP detected in | mouse kidney tissue |
Positive IHC detected in | human colon tissue, human kidney tissue, human normal colon, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
12542-1-AP targets Annexin VI in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
文献引用应用 | WB, IHC, IF, IP |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse, rat |
免疫原 | Annexin VI fusion protein Ag3263 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | annexin A6 |
别名 | Annexin A6, ANXA6, ANX6, Annexin-6, Annexin 6 |
计算分子量 | 673 aa, 76 kDa |
观测分子量 | 67 kDa |
GenBank蛋白编号 | BC017046 |
基因名称 | Annexin VI |
Gene ID (NCBI) | 309 |
RRID | AB_2057753 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P08133 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ANXA6, also called CBP68, or 67 kDa calelectrin, belongs to a family of calcium-dependent membrane and phospholipid binding proteins. Several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. The annexin VI gene is approximately 60 kbp long and contains 26 exons. It encodes a protein of about 68 kDa that consists of eight 68-amino acid repeats separated by linking sequences of variable lengths. It is highly similar to human annexins I and II sequences, each of which contain four such repeats. Annexin VI has been implicated in mediating the endosome aggregation and vesicle fusion in secreting epithelia during exocytosis.
实验方案
Product Specific Protocols | |
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WB protocol for Annexin VI antibody 12542-1-AP | Download protocol |
IHC protocol for Annexin VI antibody 12542-1-AP | Download protocol |
IF protocol for Annexin VI antibody 12542-1-AP | Download protocol |
IP protocol for Annexin VI antibody 12542-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Adv Sci (Weinh) Isolation and Comprehensive Analysis of Cochlear Tissue-Derived Small Extracellular Vesicles | ||
J Extracell Vesicles Distinguishing functional exosomes and other extracellular vesicles as a nucleic acid cargo by the anion-exchange method. | ||
Oncotarget CD300A promotes tumor progression by PECAM1, ADCY7 and AKT pathway in acute myeloid leukemia. | ||
Front Cell Dev Biol Dynamic Mitochondrial Proteome Under Polyamines Treatment in Cardiac Aging. | ||
Proteome Sci A dynamic plasma membrane proteome analysis of alcohol-induced liver cirrhosis. |