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Annexin VI Polyclonal antibody

Annexin VI Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 12542-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

Annexin A6, ANXA6, ANX6, Annexin-6, Annexin 6

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, human brain tissue, mouse brain tissue, mouse placenta tissue, mouse kidney tissue, A549 cells, Jurkat cells
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman colon tissue, human kidney tissue, human normal colon, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ANXA6 Ready-To-Use IHC Kit. ANXA6 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12542-1-AP targets Annexin VI in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原 Annexin VI fusion protein Ag3263 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 annexin A6
别名 Annexin A6, ANXA6, ANX6, Annexin-6, Annexin 6
计算分子量 673 aa, 76 kDa
观测分子量 67 kDa
GenBank蛋白编号BC017046
基因名称 Annexin VI
Gene ID (NCBI) 309
RRIDAB_2057753
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP08133
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ANXA6, also called CBP68, or 67 kDa calelectrin, belongs to a family of calcium-dependent membrane and phospholipid binding proteins. Several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. The annexin VI gene is approximately 60 kbp long and contains 26 exons. It encodes a protein of about 68 kDa that consists of eight 68-amino acid repeats separated by linking sequences of variable lengths. It is highly similar to human annexins I and II sequences, each of which contain four such repeats. Annexin VI has been implicated in mediating the endosome aggregation and vesicle fusion in secreting epithelia during exocytosis.

实验方案

Product Specific Protocols
WB protocol for Annexin VI antibody 12542-1-APDownload protocol
IHC protocol for Annexin VI antibody 12542-1-APDownload protocol
IF protocol for Annexin VI antibody 12542-1-APDownload protocol
IP protocol for Annexin VI antibody 12542-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Adv Sci (Weinh)

Isolation and Comprehensive Analysis of Cochlear Tissue-Derived Small Extracellular Vesicles

Authors - Pei Jiang
mouseWB

J Extracell Vesicles

Distinguishing functional exosomes and other extracellular vesicles as a nucleic acid cargo by the anion-exchange method.

Authors - Naohiro Seo
humanWB

Oncotarget

CD300A promotes tumor progression by PECAM1, ADCY7 and AKT pathway in acute myeloid leukemia.

Authors - Xiaogang Sun
ratWB

Front Cell Dev Biol

Dynamic Mitochondrial Proteome Under Polyamines Treatment in Cardiac Aging.

Authors - Hao Zhang
ratWB,IF

Proteome Sci

TMT-based proteomics analysis of the cerebral cortex of TauT knockout rats.

Authors - Yiming Xia
ratWB

Proteome Sci

A dynamic plasma membrane proteome analysis of alcohol-induced liver cirrhosis.

Authors - Jia Xiaofang X
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