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ANXA9 Polyclonal antibody

ANXA9 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15416-1-AP

Print datasheet

Synonyms

Annexin 31, Annexin 9, annexin A9, Annexin XXXI, ANX31, ANXA9, Pemphaxin

验证数据展示

  • WB analysis using 15416-1-AP

    WB analysis using 15416-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15416-1-AP (ANXA9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 15416-1-AP

    IHC staining of human breast cancer using 15416-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15416-1-AP (ANXA9 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 15416-1-AP

    IHC staining of human breast cancer using 15416-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15416-1-AP (ANXA9 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inA431 cells, MCF-7 cells
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue, human heart tissue, human kidney tissue, human liver tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15416-1-AP targets ANXA9 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen ANXA9 fusion protein Ag7702 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name annexin A9
Synonyms Annexin 31, Annexin 9, annexin A9, Annexin XXXI, ANX31, ANXA9, Pemphaxin
Calculated Molecular Weight 38 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC005830
Gene Symbol ANXA9
Gene ID (NCBI) 8416
RRIDAB_2057905
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO76027
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ANXA9 antibody 15416-1-APDownload protocol
IHC protocol for ANXA9 antibody 15416-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols