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AOC3 Monoclonal antibody, PBS Only

AOC3 Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

2A6G3

Cat No : 66834-1-PBS

Print datasheet

Synonyms

AOC3, Copper amine oxidase, HPAO, Membrane primary amine oxidase, SSAO, VAP 1, VAP1, Vascular adhesion protein 1

验证数据展示

  • WB analysis of human ileum using 66834-1-Ig (same clone as 66834-1-PBS)

    WB analysis of human ileum using 66834-1-Ig (same clone as 66834-1-PBS)

    human ileum tissue were subjected to SDS PAGE followed by western blot with 66834-1-Ig (AOC3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66834-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66834-1-Ig (same clone as 66834-1-PBS)

    IHC staining of human liver cancer using 66834-1-Ig (same clone as 66834-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66834-1-Ig (AOC3 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66834-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66834-1-Ig (same clone as 66834-1-PBS)

    IHC staining of human liver cancer using 66834-1-Ig (same clone as 66834-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66834-1-Ig (AOC3 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66834-1-PBS in a different storage buffer formulation.

  • IF Staining of human liver cancer using 66834-1-Ig (same clone as 66834-1-PBS)

    IF Staining of human liver cancer using 66834-1-Ig (same clone as 66834-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using AOC3 antibody (66834-1-Ig, Clone: 2A6G3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66834-1-PBS in a different storage buffer formulation.

  • IF Staining of human liver cancer using 66834-1-Ig (same clone as 66834-1-PBS)

    IF Staining of human liver cancer using 66834-1-Ig (same clone as 66834-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using AOC3 antibody (66834-1-Ig, Clone: 2A6G3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66834-1-PBS in a different storage buffer formulation.

产品信息

66834-1-PBS targets AOC3 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB,Indirect ELISA,IHC,IF Application Description
Tested Reactivity Human
Immunogen AOC3 fusion protein Ag5908 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name amine oxidase, copper containing 3 (vascular adhesion protein 1)
Synonyms AOC3, Copper amine oxidase, HPAO, Membrane primary amine oxidase, SSAO, VAP 1, VAP1, Vascular adhesion protein 1
Calculated Molecular Weight 85 kDa
Observed Molecular Weight 85-90 kDa
GenBank Accession NumberBC050549
Gene Symbol AOC3
Gene ID (NCBI) 8639
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ16853
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

AOC3(membrane primary amine oxidase) is also name as copper amine oxidase, HPAO, SSAO, VAP1 and belongs to the copper/topaquinone oxidase family. It is expressed on the surface of endothelial cells and is involved in leukocyte trafficking between blood and tissues under physiologic and pathologic conditions (PMID:17947691). AOC3 is highly expressed in adipocytes and smooth muscle cells and it can be N- and O-glycosylated (PMID:16046623). AOC3 has molecular mass of 70-90kD, and can exsit as a homodimer(165-185kD) and trimer(240~260kD) (PMID:10595925, 8625974).