验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue, mouse lung tissue, mouse pancreas tissue |
| Positive IP detected in | mouse lung tissue |
| Positive IHC detected in | human hepatocirrhosis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
19495-1-AP targets Aldehyde oxidase in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat, canine |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | aldehyde oxidase 1 |
| 别名 | EC:1.2.3.1, EC:1.17.3.-, Azaheterocycle hydroxylase, AOX1, AOX 1 |
| 计算分子量 | 148 kDa |
| 观测分子量 | 148 kDa |
| GenBank蛋白编号 | NM_001159 |
| 基因名称 | AOX1 |
| Gene ID (NCBI) | 316 |
| RRID | AB_10640579 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q06278 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Aldehyde oxidase antibody 19495-1-AP | Download protocol |
| IHC protocol for Aldehyde oxidase antibody 19495-1-AP | Download protocol |
| IF protocol for Aldehyde oxidase antibody 19495-1-AP | Download protocol |
| IP protocol for Aldehyde oxidase antibody 19495-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
EMBO Rep Deficiency in mTORC1-controlled C/EBPβ-mRNA translation improves metabolic health in mice. | ||
Oncogene Epigenetic loss of AOX1 expression via EZH2 leads to metabolic deregulations and promotes bladder cancer progression.
| ||
Free Radic Biol Med PPARα agonist WY-14,643 enhances ethanol metabolism in mice: role of catalase. | ||
Front Immunol Insight of a Metabolic Prognostic Model to Identify Tumor Environment and Drug Vulnerability for Lung Adenocarcinoma. | ||
Cell Signal Aldehyde oxidase 1 promotes gallbladder carcinogenesis through ROS-mediated activation of the Wnt/β-catenin pathway |
