验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, mouse skeletal muscle tissue |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 5 publications below |
| IF | See 2 publications below |
产品信息
13258-1-AP targets Gamma Adaptin in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | Gamma Adaptin fusion protein Ag3696 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | adaptor-related protein complex 1, gamma 1 subunit |
| 别名 | ADTG, AP 1 complex subunit gamma 1, AP1G1, CLAPG1, Gamma1 adaptin |
| 计算分子量 | 825 aa, 92 kDa |
| 观测分子量 | 91 kDa |
| GenBank蛋白编号 | BC036283 |
| 基因名称 | Gamma Adaptin |
| Gene ID (NCBI) | 164 |
| RRID | AB_2058209 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O43747 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Gamma Adaptin antibody 13258-1-AP | Download protocol |
| IHC protocol for Gamma Adaptin antibody 13258-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Molecular identification of a BAR domain-containing coat complex for endosomal recycling of transmembrane proteins. | ||
Proc Natl Acad Sci U S A An in vitro vesicle formation assay reveals cargo clients and factors that mediate vesicular trafficking. | ||
Front Cell Dev Biol Visualization of Protein Sorting at the Trans-Golgi Network and Endosomes Through Super-Resolution Imaging. | ||
Cells Five Inhibitory Receptors Display Distinct Vesicular Distributions in Murine T Cells | ||
Front Cell Neurosci MEA6 Deficiency Impairs Cerebellar Development and Motor Performance by Tethering Protein Trafficking. | ||
