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  • Featured Product
  • KD/KO Validated

AP1M1 Recombinant antibody, PBS Only

AP1M1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240553H3

Cat no : 83613-6-PBS

Print datasheet

Synonyms

AP-1 complex subunit mu-1, AP mu chain family member mu1A, AP 1 complex subunit mu 1, Adaptor-related protein complex 1 subunit mu-1, Adaptor protein complex AP-1 subunit mu-1

验证数据展示

  • WB analysis using 83613-6-RR (same clone as 83613-6-PBS)

    WB analysis using 83613-6-RR (same clone as 83613-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83613-6-RR (AP1M1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83613-6-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 83613-6-RR (same clone as 83613-6-PBS)

    WB analysis of HEK-293 using 83613-6-RR (same clone as 83613-6-PBS)

    WB result of AP1M1 antibody (83613-6-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AP1M1 transfected HEK-293 cells. This data was developed using the same antibody clone with 83613-6-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83613-6-RR (same clone as 83613-6-PBS)

    FC experiment of HeLa using 83613-6-RR (same clone as 83613-6-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug AP1M1 Recombinant antibody (83613-6-RR, Clone:240553H3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83613-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83613-6-RR

    Affinity and Kinetic Characterization of 83613-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83613-6-RR against Human AP1M1 were performed. The affinity constant is below 1 pM.

产品信息

83613-6-PBS targets AP1M1 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen AP1M1 fusion protein Ag2757 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name adaptor-related protein complex 1, mu 1 subunit
Synonyms AP-1 complex subunit mu-1, AP mu chain family member mu1A, AP 1 complex subunit mu 1, Adaptor-related protein complex 1 subunit mu-1, Adaptor protein complex AP-1 subunit mu-1
Calculated Molecular Weight 423 aa, 49 kDa
Observed Molecular Weight 49 kDa
GenBank Accession NumberBC017469
Gene Symbol AP1M1
Gene ID (NCBI) 8907
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9BXS5
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

AP1M1, also named as CLTNM, Mu-adaptin 1 and Clathrin coat assembly protein AP47, belongs to the adaptor complexes medium subunit family. It is a subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the trans-Golgi network (TGN) and endosomes. The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.