验证数据展示
经过测试的应用
| Positive WB detected in | rat epididymis tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15043-1-AP targets AP1S2 in WB, ELISA applications and shows reactivity with human, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, rat |
| 免疫原 | AP1S2 fusion protein Ag6911 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | adaptor-related protein complex 1, sigma 2 subunit |
| 别名 | AP 1 complex subunit sigma 2, AP1S2, DC22, MRX59, Sigma adaptin 1B, SIGMA1B, Sigma1B adaptin |
| 计算分子量 | 19 kDa |
| 观测分子量 | 19-24 kDa |
| GenBank蛋白编号 | BC001117 |
| 基因名称 | AP1S2 |
| Gene ID (NCBI) | 8905 |
| RRID | AB_3669197 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P56377 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
AP1S2 is a subunit of the adaptor complex AP-1. It belongs to the adaptor complexes small subunit family. Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP-1 is found at the cytoplasmic face of coated vesicles located at the Golgi complex, where it mediates both the recruitment of clathrin to the membrane and the recognition of sorting signals within the cytosolic tails of transmembrane receptors.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for AP1S2 antibody 15043-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |