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验证数据展示

  • IHC staining of mouse stomach using 17365-1-AP

    IHC staining of mouse stomach using 17365-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 17365-1-AP (AP1S3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse stomach using 17365-1-AP

    IHC staining of mouse stomach using 17365-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 17365-1-AP (AP1S3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

AP1S3 Polyclonal antibody

AP1S3 Polyclonal Antibody for IHC, ELISA
Cat No. 17365-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, ELISA

别名

Sigma1C-adaptin, Sigma 1C subunit of AP-1 clathrin, Golgi adaptor HA1/AP1 adaptin sigma-1C subunit, Clathrin assembly protein complex 1 sigma-1C small chain, Adaptor-related protein complex 1 subunit sigma-1C

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inmouse stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17365-1-AP targets AP1S3 in IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 AP1S3 fusion protein Ag10751 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 adaptor-related protein complex 1, sigma 3 subunit
别名 Sigma1C-adaptin, Sigma 1C subunit of AP-1 clathrin, Golgi adaptor HA1/AP1 adaptin sigma-1C subunit, Clathrin assembly protein complex 1 sigma-1C small chain, Adaptor-related protein complex 1 subunit sigma-1C
计算分子量104aa,11 kDa; 154aa,18 kDa
GenBank蛋白编号BC021898
基因名称 AP1S3
Gene ID (NCBI) 130340
RRIDAB_3085522
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96PC3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AP1S3 is a subunit of the adaptor complex AP-1. It belongs to the adaptor complexes small subunit family. Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP-1 is found at the cytoplasmic face of coated vesicles located at the Golgi complex, where it mediates both the recruitment of clathrin to the membrane and the recognition of sorting signals within the cytosolic tails of transmembrane receptors.

实验方案

Product Specific Protocols
IHC protocol for AP1S3 antibody 17365-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols