AP2A1 Polyclonal antibody
AP2A1 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | A431 cells, HEK-293 cells, MCF-7 cells, SKOV-3 cells, SH-SY5Y cells, mouse kidney tissue, rat kidney tissue, mouse kidney cells, rat kidney cells |
Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:2000-1:12000 |
Immunohistochemistry (IHC) | IHC : 1:600-1:2400 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
30030-1-AP targets AP2A1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, ELISA Application Description |
Tested Reactivity | human, mouse, rat |
Immunogen | AP2A1 fusion protein Ag32232 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | adaptor-related protein complex 2, alpha 1 subunit |
Synonyms | Alpha adaptin A, alpha Adaptin, ADTAA, Adaptor-related protein complex 2 subunit alpha-1, Adaptor protein complex AP-2 subunit alpha-1 |
Calculated Molecular Weight | 977 aa, 108 kDa |
Observed Molecular Weight | 108 kDa |
GenBank Accession Number | BC014214 |
Gene Symbol | AP2A1 |
Gene ID (NCBI) | 160 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | O95782 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for AP2A1 antibody 30030-1-AP | Download protocol |
IHC protocol for AP2A1 antibody 30030-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |