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验证数据展示

  • WB analysis using 30030-1-AP

    WB analysis using 30030-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30030-1-AP (AP2A1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 30030-1-AP

    IHC staining of human stomach cancer using 30030-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 30030-1-AP (AP2A1 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with AP2A1 antibody included

AP2A1 Polyclonal antibody

AP2A1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30030-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Alpha adaptin A, alpha Adaptin, ADTAA, Adaptor-related protein complex 2 subunit alpha-1, Adaptor protein complex AP-2 subunit alpha-1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, HEK-293 cells, MCF-7 cells, SKOV-3 cells, SH-SY5Y cells, mouse kidney tissue, rat kidney tissue, mouse kidney cells, rat kidney cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy AP2A1 Ready-To-Use IHC Kit. AP2A1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:600-1:2400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30030-1-AP targets AP2A1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 AP2A1 fusion protein Ag32232 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 adaptor-related protein complex 2, alpha 1 subunit
别名 Alpha adaptin A, alpha Adaptin, ADTAA, Adaptor-related protein complex 2 subunit alpha-1, Adaptor protein complex AP-2 subunit alpha-1
计算分子量 977 aa, 108 kDa
观测分子量108 kDa
GenBank蛋白编号BC014214
基因名称 AP2A1
Gene ID (NCBI) 160
RRIDAB_2935505
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95782
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AP2A1 encodes adaptor protein complex 2 (AP-2) subunit alpha-1, which is a 100 kDa coated vesicle protein. Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP complexes are involved in the formation of clathrin-coated vesicles (CCVs) by recruiting the scaffold protein, clathrin. AP complexes also play a pivotal role in the cargo selection by recognizing the sorting signals within the cytoplasmic tail of integral membrane proteins. AP-2 is involved in clathrin-dependent endocytosis.

实验方案

Product Specific Protocols
WB protocol for AP2A1 antibody 30030-1-APDownload protocol
IHC protocol for AP2A1 antibody 30030-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols