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验证数据展示

  • WB analysis of mouse brain using 15722-1-AP

    WB analysis of mouse brain using 15722-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 15722-1-AP (AP2A2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 15722-1-AP

    IHC staining of human testis using 15722-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 15722-1-AP (AP2A2 Antibody) at dilution of 1:50 (under 40x lens).

AP2A2 Polyclonal antibody

AP2A2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15722-1-AP

Print datasheet

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Synonyms

ADTAB, Alpha adaptin C, Alpha2 adaptin, AP 2 complex subunit alpha 2, AP2A2, CLAPA2, HIP 9, HIP9, Huntingtin yeast partner J, HYPJ, KIAA0899

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

15722-1-AP targets AP2A2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitypig
Immunogen AP2A2 fusion protein Ag8442 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name adaptor-related protein complex 2, alpha 2 subunit
Synonyms ADTAB, Alpha adaptin C, Alpha2 adaptin, AP 2 complex subunit alpha 2, AP2A2, CLAPA2, HIP 9, HIP9, Huntingtin yeast partner J, HYPJ, KIAA0899
Calculated Molecular Weight 939 aa, 104 kDa
Observed Molecular Weight 104 kDa
GenBank Accession NumberBC006155
Gene Symbol AP2A2
Gene ID (NCBI) 161
RRIDAB_2878175
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO94973
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AP2A2, also named as ADTAB, CLAPA2, HIP9, HYPJ, or KIAA0899, is a 939 amino acid protein, which belongs to the adaptor complexes large subunit family. AP2A2 localizes in the membrane and is a component of the adaptor protein complex 2. AP2 adaptor complex linking clathrin to receptors in coated vesicles, clathrin-associated protein complexes are believed to interact with cytoplasmic domains of transmembrane proteins leading to their selection and concentration. AP2A2 is invovled into cellular trafficking transport.

实验方案

Product Specific Protocols
WB protocol for AP2A2 antibody 15722-1-APDownload protocol
IHC protocol for AP2A2 antibody 15722-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
pigWB

Emerg Microbes Infect

CD1d facilitates African swine fever virus entry into the host cells via clathrin-mediated endocytosis

Authors - Xin Chen
View Article