验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, SH-SY5Y cells, K-562 cells, C6 cells, NIH/3T3 cells |
| Positive IP detected in | K-562 cells |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15690-1-AP targets AP2B1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, pig, monkey |
| 免疫原 | AP2B1 fusion protein Ag8248 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | adaptor-related protein complex 2, beta 1 subunit |
| 别名 | Beta-2-adaptin, AP-2 complex subunit beta, AP 2 complex subunit beta, ADTB2, Adaptor-related protein complex 2 subunit beta |
| 计算分子量 | 105 kDa |
| 观测分子量 | 100-105 kDa |
| GenBank蛋白编号 | BC006201 |
| 基因名称 | AP2B1 |
| Gene ID (NCBI) | 163 |
| RRID | AB_2056351 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P63010 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
AP2B1 is a component of the adaptor protein complex 2 (AP-2). AP complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP complexes are involved in the formation of clathrin-coated vesicles (CCVs) by recruiting the scaffold protein, clathrin. AP complexes also play a pivotal role in the cargo selection by recognizing the sorting signals within the cytoplasmic tail of integral membrane proteins. AP-2 works on the plasma membrane to internalize cargo in clathrin-mediated endocytosis.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for AP2B1 antibody 15690-1-AP | Download protocol |
| IF protocol for AP2B1 antibody 15690-1-AP | Download protocol |
| IP protocol for AP2B1 antibody 15690-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Emerg Microbes Infect CD1d facilitates African swine fever virus entry into the host cells via clathrin-mediated endocytosis | ||
Elife Syncytin-mediated open-ended membrane tubular connections facilitate the intercellular transfer of cargos including Cas9 protein | ||
PLoS Pathog Pseudorabies virus inhibits progesterone-induced inactivation of TRPML1 to facilitate viral entry | ||
Sci China Life Sci NPC1-regulated dynamic of clathrin-coated pits is essential for viral entry. | ||
J Virol The G protein-coupled receptor FFAR2 promotes internalization during influenza A virus entry.
| ||
Viruses Pseudorabies Virus Inhibits Expression of Liver X Receptors to Assist Viral Infection. |
