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AP2B1 Monoclonal antibody, PBS Only

AP2B1 Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat, Rabbit, Pig

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

1A3B6

Cat No : 68349-1-PBS

Print datasheet

Synonyms

ADTB2, AP 2 complex subunit beta, AP105B, AP2 BETA, AP2B1, Beta 2 adaptin, Beta adaptin, CLAPB1, DKFZp781K0743

验证数据展示

  • WB analysis using 68349-1-Ig (same clone as 68349-1-PBS)

    WB analysis using 68349-1-Ig (same clone as 68349-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68349-1-Ig (AP2B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68349-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 68349-1-Ig (same clone as 68349-1-PBS)

    IHC staining of rat brain using 68349-1-Ig (same clone as 68349-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68349-1-Ig (AP2B1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68349-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 68349-1-Ig (same clone as 68349-1-PBS)

    IHC staining of rat brain using 68349-1-Ig (same clone as 68349-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68349-1-Ig (AP2B1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68349-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 68349-1-Ig (same clone as 68349-1-PBS)

    IF Staining of HeLa using 68349-1-Ig (same clone as 68349-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using AP2B1 antibody (68349-1-Ig, Clone: 1A3B6 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68349-1-PBS in a different storage buffer formulation.

产品信息

68349-1-PBS targets AP2B1 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit, Pig samples.

Tested Applications WB,Indirect ELISA,IHC,IF Application Description
Tested Reactivity Human, Mouse, Rat, Rabbit, Pig
Immunogen AP2B1 fusion protein Ag8264 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name adaptor-related protein complex 2, beta 1 subunit
Synonyms ADTB2, AP 2 complex subunit beta, AP105B, AP2 BETA, AP2B1, Beta 2 adaptin, Beta adaptin, CLAPB1, DKFZp781K0743
Calculated Molecular Weight 105 kDa
Observed Molecular Weight100-115 kDa
GenBank Accession NumberBC006201
Gene Symbol AP2B1
Gene ID (NCBI) 163
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP63010
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

AP2B1 is a component of the adaptor protein complex 2 (AP-2). AP complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP complexes are involved in the formation of clathrin-coated vesicles (CCVs) by recruiting the scaffold protein, clathrin. AP complexes also play a pivotal role in the cargo selection by recognizing the sorting signals within the cytoplasmic tail of integral membrane proteins. AP-2 works on the plasma membrane to internalize cargo in clathrin-mediated endocytosis.