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Host
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验证数据展示

  • WB analysis using 84174-3-RR (same clone as 84174-3-PBS)

    WB analysis using 84174-3-RR (same clone as 84174-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84174-3-RR (AP2S1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84174-3-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 84174-3-RR (same clone as 84174-3-PBS)

    IF Staining of HeLa using 84174-3-RR (same clone as 84174-3-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using AP2S1 antibody (84174-3-RR, Clone: 241369F1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84174-3-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 84174-3-RR (same clone as 84174-3-PBS)

    IHC staining of mouse testis using 84174-3-RR (same clone as 84174-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84174-3-RR (AP2S1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84174-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84174-3-RR

    Affinity and Kinetic Characterization of 84174-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84174-3-RR against Human AP2S1 were performed. The affinity constant is 0.502 nM.

AP2S1 Recombinant antibody, PBS Only

AP2S1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 84174-3-PBS

产品说明书

CloneNo. 241369F1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

AP17 DELTA, AP17, AP 2 complex subunit sigma, Adaptor-related protein complex 2 subunit sigma, Adaptor protein complex AP-2 subunit sigma

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84174-3-PBS targets AP2S1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 AP2S1 fusion protein Ag8095 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 adaptor-related protein complex 2, sigma 1 subunit
别名 AP17 DELTA, AP17, AP 2 complex subunit sigma, Adaptor-related protein complex 2 subunit sigma, Adaptor protein complex AP-2 subunit sigma
计算分子量 142 aa, 17 kDa
观测分子量15-17 kDa
GenBank蛋白编号BC006337
基因名称 AP2S1
Gene ID (NCBI) 1175
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP53680
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

AP2S1 is a component of the adaptor protein complex 2 (AP-2). AP complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP complexes form clathrin-coated vesicles (CCVs) by recruiting the scaffold protein, clathrin. AP complexes also play a pivotal role in cargo selection by recognizing the sorting signals within the cytoplasmic tail of integral membrane proteins. AP-2 is composed of two large adaptins (alpha-type subunit AP2A1 or AP2A2 and beta-type subunit AP2B1), a medium adaptin (mu-type subunit AP2M1), and a small adaptin (sigma-type subunit AP2S1). It works on the plasma membrane to internalize cargo in clathrin-mediated endocytosis. Missense mutations of AP2S1 affect Arg15 and lead to familial hypocalciuric hypercalcemia type 3 (FHH3), an extracellular calcium homeostasis disorder affecting the parathyroids, kidneys, and bone (PMID: 23222959).