验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, HeLa cells, NIH/3T3 cells, HepG2 cells |
| Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IF | See 3 publications below |
产品信息
16454-1-AP targets AP3D1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | mouse, rat |
| 免疫原 | AP3D1 fusion protein Ag9390 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | adaptor-related protein complex 3, delta 1 subunit |
| 别名 | ADTD, AP 3 complex subunit delta, AP 3 complex subunit delta 1, AP3D1, Delta adaptin, hBLVR |
| 计算分子量 | 1153 aa, 130 kDa |
| 观测分子量 | 110 kDa |
| GenBank蛋白编号 | BC010065 |
| 基因名称 | AP3D1 |
| Gene ID (NCBI) | 8943 |
| RRID | AB_10596951 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O14617 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP3D1 is a subunit of the AP-3 complex which is composed of two large adaptins (AP3D1 and AP3B1 or AP3B2), a medium adaptin (AP3M1 or AP3M2) and a small adaptin (APS1 or AP3S2). AP-3 complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for AP3D1 antibody 16454-1-AP | Download protocol |
| IF protocol for AP3D1 antibody 16454-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Discov Loss of Optineurin Drives Cancer Immune Evasion via Palmitoylation-Dependent IFNGR1 Lysosomal Sorting and Degradation. | ||
Cell Rep Defective endomembrane dynamics in Rab27a deficiency impairs nucleic acid sensing and cytokine secretion in immune cells |
