验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, NIH/3T3 cells |
| Positive IHC detected in | mouse cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
产品信息
12114-1-AP targets AP3M1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, rat |
| 免疫原 | AP3M1 fusion protein Ag2759 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | adaptor-related protein complex 3, mu 1 subunit |
| 别名 | Mu-adaptin 3A, Mu3A-adaptin, AP-3 complex subunit mu-1, AP-3 adaptor complex mu3A subunit, Adaptor-related protein complex 3 subunit mu-1 |
| 计算分子量 | 418 aa, 47 kDa |
| 观测分子量 | 47 kDa |
| GenBank蛋白编号 | BC026232 |
| 基因名称 | AP3M1 |
| Gene ID (NCBI) | 26985 |
| RRID | AB_2289629 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y2T2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP3M1 is a subunit of the AP-3 complex which is composed of two large adaptins (AP3D1 and AP3B1 or AP3B2), a medium adaptin (AP3M1 or AP3M2) and a small adaptin (APS1 or AP3S2). AP-3 complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for AP3M1 antibody 12114-1-AP | Download protocol |
| IHC protocol for AP3M1 antibody 12114-1-AP | Download protocol |
| IF protocol for AP3M1 antibody 12114-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Elife A novel GTP-binding protein-adaptor protein complex responsible for export of Vangl2 from the trans Golgi network.
| ||
J Ethnopharmacol Zhachong Shisanwei Pill resists ischemic stroke by lysosome pathway based on proteomics and bioinformatics |
