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AP3M2 Polyclonal antibody

AP3M2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11925-1-AP

Print datasheet

Synonyms

AP 3 complex subunit mu 2, AP3M2, AP47B, CLA20, HA1 47 kDa subunit homolog 2, Mu3B adaptin, P47B

验证数据展示

  • WB analysis of Hacat cells (siRNA) using 11925-1-AP

    WB analysis of Hacat cells (siRNA) using 11925-1-AP

    WB result of AP3M2 antibody (11925-1-AP; 1:500) with siRNA cell lysate.

  • WB analysis of human brain using 11925-1-AP

    WB analysis of human brain using 11925-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 11925-1-AP (AP3M2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 11925-1-AP

    IP experiment of mouse brain using 11925-1-AP

    IP result of anti-AP3M2 (IP:11925-1-AP, 4ug; Detection:11925-1-AP 1:300) with mouse brain tissue lysate 3200 ug.

  • IHC staining of mouse brain using 11925-1-AP

    IHC staining of mouse brain using 11925-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11925-1-AP (AP3M2 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of mouse brain using 11925-1-AP

    IHC staining of mouse brain using 11925-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11925-1-AP (AP3M2 Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inhuman brain tissue, Hacat cells (siRNA)
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11925-1-AP targets AP3M2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen AP3M2 fusion protein Ag2572 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name adaptor-related protein complex 3, mu 2 subunit
Synonyms AP 3 complex subunit mu 2, AP3M2, AP47B, CLA20, HA1 47 kDa subunit homolog 2, Mu3B adaptin, P47B
Calculated Molecular Weight 418 aa, 47 kDa
Observed Molecular Weight 47 kDa
GenBank Accession NumberBC056257
Gene Symbol AP3M2
Gene ID (NCBI) 10947
RRIDAB_10640438
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP53677
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AP3M2 is a 47-kDa protein that belongs to the adaptor complexes medium subunit family. Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP3M2 is a subunit of the AP-3 complex which is associated with the Golgi region as well as more peripheral structures. AP-3 is implicated in the transport of lysosomal membrane proteins.

实验方案

Product Specific Protocols
WB protocol for AP3M2 antibody 11925-1-APDownload protocol
IHC protocol for AP3M2 antibody 11925-1-APDownload protocol
IP protocol for AP3M2 antibody 11925-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols